Method for altering the isoflavonoid profile in the plant parts of an isoflavonoid-producing plant
OwnerJoan T. Odell, Xiaodan Yuinventors
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Abstract
A method for altering the ratio of total daidzein to total genistein in isoflavonoid-producing plants by using a C1 myb transcription factor and an R-type myc transcription factor is described. Also described are plant comprising these transcription factors in their genome as well as isoflavonoid-containing products made from seeds of these plants. Such products have an increased ratio of total daidzein to total genistein when compared to the total daidzein to total genistein ratio of a control.
Claims52
A method of altering the isoflavonoid profile of an isoflavonoid-producing plant, said method comprising:
- (a) transforming a plant with (i) a first recombinant expression construct comprising a promoter operably linked to an isolated nucleic acid fragment encoding a C1 myb transcription factor and a recombinant expression construct comprising a promoter operably linked to an isolated nucleic acid fragment encoding an R myc-type transcription factor, (ii) a second recombinant expression construct comprising a promoter operably linked to an isolated nucleic acid fragment encoding a C1 myb transcription factor and a promoter operably linked to an isolated nucleic acid fragment encoding an R myc-type transcription factor, or (iii) a recombinant expression construct comprising a promoter operably linked to an isolated nucleic acid fragment encoding all or part of a C1 myb transcription factor and all or part of an R myc-type wherein said construct is capable of functioning as both a C1 myb transcription factor and an R myc-type transcription factor;
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- (b) growing the transformed plant under conditions that are suitable for the expression of the recombinant expression construct or constructs;
- wherein expression of the construct or constructs alters the isoflavonoid profile of the transformed plant by increasing the total daidzein to total genistein ratio compared to the total daidzein to total genistein ratio of a control.
An isoflavonoid-producing plant comprising in its genome
- (i) a first recombinant expression construct comprising a promoter operably linked to an isolated nucleic acid fragment encoding a C1 myb transcription factor and a recombinant expression construct comprising a promoter operably linked to an isolated nucleic acid fragment encoding an R myc-type transcription factor,
- (ii) a second recombinant expression construct comprising a promoter operably linked to an isolated nucleic acid fragment encoding a C1 myb transcription factor and a promoter operably linked to an isolated nucleic acid fragment encoding an R myc-type transcription factor, or
- (iii) a recombinant expression construct comprising a promoter operably linked to an isolated nucleic acid fragment encoding all or part of a C1 myb transcription factor and all or part of an R myc-type wherein said construct is capable of functioning as both a C1 myb transcription factor and an R myc-type transcription factor;
- wherein said plant has an increased total daidzein to total genistein ratio when compared to the total daidzein to total genistein ratio of a control.
Dependencies are inferred from the claim text.