Transgenic plants with altered levels of phenolic compounds
AssigneeThe Ohio State University Research Foundation
InventorsErich Grotewold, Anusha P. Dias, Edward L. Braun
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AssigneeThe Ohio State University Research Foundation
InventorsErich Grotewold, Anusha P. Dias, Edward L. Braun
Estimating the term…
Methods for altering levels in plants of one or more phenolic compounds that are intermediates or final products of the plant phenylpropanoid pathway are provided. One method comprises transforming a plant cell with an expression construct comprising a nucleic acid which encodes a transactivator protein comprising the myb domain of the maize “ZmMyb-IF35” protein and an activation domain. Another method comprises transforming a plant cell with an expression construct comprising a transgene which encodes an antisense ZmMyb-IF35 RNA. The present invention also relates to expression constructs and vectors used in the present methods. transformed plant cells and transgenic plants prepared according to the present methods, and the seeds of such transgenic plants.
An expression construct for preparing plant cells with altered levels of one or more phenolic compounds that are intermediates or final products of the plant phenylpropanoid pathway, comprising:
An expression construct for preparing a plant with altered levels of one or more phenolic compounds of the phenylpropanid pathway of the plant comprising:
An expression construct that, when contained in a plant cell, generates RNA which is sufficiently complementary to an RNA transcribed from an endogenous ZmMyb-IF35 gene to prevent synthesis of endogenous ZmMyb-IF35 protein in the plant cell, wherein said expression construct comprises the reverse transcript of said complementary RNA operably linked to a promoter which effects its transcription into said complementary RNA, and wherein said endogenous ZmMyb-IF35 protein comprises a sequence which comprises amino acid 1 through amino acid 345 of SEQ ID NO. 2.
Isolated DNA or RNA comprising a sequence which
A transgenic plant, plant part, plant cell, or tissue culture, each of which is transformed with an isolated nucleic acid comprising a sequence selected from the group consisting of
An expression construct for preparing a plant with altered levels of one or more phenolic compounds of the phenylpropanid pathway of the plant comprising
Dependencies are inferred from the claim text.