Barley gene for thioredoxin and NADP-thioredoxin reductase
AssigneeThe Regents of the University of California
InventorsMyeong-Je Cho, Gregorio del Val, Maxime Caillau, Peggy G. Lemaux, Bob B. Buchanan
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Abstract
The present invention provides barley thioredoxin h nucleic acids and NADP-thioredoxin reductase nucleic acids, the respective encoded proteins and methods of use.
Claims72
A recombinant nucleic acid encoding a thioredoxin h protein comprising a nucleic acid that hybridizes under high stringency conditions to a sequence complementary to that set forth in FIG. 2 (SEQ ID NO:1).
A recombinant nucleic acid encoding a thioredoxin h protein comprising a nucleic acid having at least 75% sequence identity to a sequence as set forth in FIG. 2 (SEQ ID NO:1).
A recombinant nucleic acid encoding an amino acid sequence as shown in FIG. 1 (SEQ ID NO:2).
A recombinant thioredoxin h polypeptide comprising an amino acid sequence having at least 80% sequence identity with the sequence set forth in FIG. 15 (SEQ ID NO:25).
A method of identifying a bioactive agent that binds to a thioredoxin h polypeptide comprising an amino acid sequence having at least 80% sequence identity with the sequence set forth in FIG. 1 (SEQ ID NO:2), said method comprising:
- a) combining said thioredoxin h polypeptide and a candidate bioactive agent;
- and
- b) determining the binding of said candidate bioactive agent to said thioredoxin h polypeptide, whereby said bioactive agent is identified.
A method of identifying a bioactive agent that modulates an activity of a thioredoxin h polypeptide comprising an amino acid sequence having at least 80% sequence identity with the sequence set forth in FIG. 1 (SEQ ID NO:2), said method comprising;
- a) combining said thioredoxin h polypeptide and a candidate bioactive agent(s);
- and
- b) determining the effect of said candidate bioactive agent(s) on an activity of said thioredoxin h polypeptide.
A recombinant nucleic acid encoding an NTR protein comprising a nucleic acid that hybridizes under high stringency conditions to a sequence complementary to that set forth in FIG. 5A (SEQ ID NO:10).
A recombinant nucleic acid encoding an NTR protein comprising a nucleic acid having at least 75% sequence identity to a sequence as set forth in FIG. 5A (SEQ ID NO:10).
A recombinant nucleic acid encoding an amino acid sequence as shown in FIG. 4 (SEQ ID NO:9).
A recombinant NTR polypeptide comprising an amino acid sequence having at least 80% sequence identity with the sequence set forth in FIG. 4 (SEQ ID NO:9).
A method of identifying a bioactive agent that binds to an NTR polypeptide comprising an amino acid sequence having at least 80% sequence identity with the sequence set forth in FIG. 4 (SEQ ID NO:9), said method comprising:
- a) combining said NTR polypeptide and a candidate bioactive agent;
- and
- b) determining the binding of said candidate bioactive agent to said NTR polypeptide, whereby said bioactive agent is identified.
A method of identifying a bioactive agent that modulates an activity of an NTR polypeptide comprising an amino acid sequence having at least 80% sequence identity with the sequence set forth in FIG. 4 (SEQ ID NO:9), said method comprising;
- a) combining said NTR polypeptide and a candidate bioactive agent(s);
- and
- b) determining the effect of said candidate bioactive agent(s) on an activity of said NTR polypeptide.
An isolated nucleic acid encoding a thioredoxin h protein comprising a nucleic acid that hybridizes under high stringency conditions to a sequence complementary to that set forth in FIG. 2 (SEQ ID NO:1).
An isolated nucleic acid encoding a thioredoxin h protein comprising a nucleic acid having at least 75% sequence identity to a sequence as set forth in FIG. 2 (SEQ ID NO:1).
An isolated nucleic acid encoding an amino acid sequence as shown in FIG. 1 (SEQ ID NO:2).
A isolated nucleic acid encoding an NTR protein comprising a nucleic acid that hybridizes under high stringency conditions to a sequence complementary to that set forth in FIG. 5A (SEQ ID NO:10).
An isolated nucleic acid encoding an NTR protein comprising a nucleic acid having at least 75% sequence identity to a sequence as set forth in FIG. 5A (SEQ ID NO:10).
An isolated nucleic acid encoding an amino acid sequence as shown in FIG. 4 (SEQ ID NO:9).
A transgenic plant or at least a part of said plant overexpressing a barley thioredoxin h protein.
A transgenic plant or at least a part of said plant overexpressing a barley NADP-thioredoxin reductase protein.
Dependencies are inferred from the claim text.