Method of generating transgenic organisms using transposons
OwnerCharalambos Savakis, Frank Grosveldinventors
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Abstract
The invention relates to a method for generating a transgenic organism. The invention also relates to a method for detecting and characterizing a genetic mutation in a transgenic organism. The invention further relates to a method for isolating a gene which is correlated with a phenotypic characteristic in a transgenic animal. The invention further relates to a method for isolating an exon in a transgenic animal. The invention also relates to a method for modulating the expression of a gene in an organism.
Claims29
A method for generating a transgenic organism, comprising the steps of:
- (a) providing a first transgenic organism, which organism comprises, within at least a portion of its tissues or cells, one or more copies of a Minos transposon;
- (b) providing a second organism, which organism comprises, in the genome of at least a portion of its tissues or cells, a Minos transposase or one or more copies of a gene encoding a Minos transposase;
- and
- (c) crossing the organism so as to obtain transgenic progeny which comprise, in at least a portion of their tissues or cells, both the Minos transposon and the Minos transposase.
A method for generating a transgenic organism, comprising the steps of:
- (a) providing a first transgenic organism, which organism comprises, within at least a portion of its tissues or cells, one or more copies of a transposon,
- (b) providing a second organism, which organism comprises, in the genome of at least a portion of its tissues or cells, a transposase or one or more copies of a gene encoding a transposase;
- and
- (c) crossing the organism so as to obtain transgenic progeny which comprise, in at least a portion of their tissues or cells, both the transposon and the transposase.
A transgenic organism comprising one or more copies of a heterologous transposon, said transgenic organism being free of nucleic acid sequences encoding the cognate transposase enzyme.
A transgenic organism encoding a transposase enzyme, said transgenic organism being free of the cognate transposon.
Dependencies are inferred from the claim text.