Transgenic animals expressing light-emitting fusion proteins and diagnostic and therapeutic methods therefor
AssigneeDana-Farber Cancer Institute, Inc.
InventorsWilliam G. Kaelin JR., David M. Livingston, Tae-You Kim
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Abstract
Light-generating fusion proteins having a ligand binding site and a light-generating polypeptide moiety and their use as diagnostics, in drug screening and discovery, and as therapeutics, are disclosed. The light-generating fusion protein has a feature where the bioluminescence of the polypeptide moiety changes upon binding of a ligand at the ligand binding site. The ligand may be, for example, an enzyme present in an environment only under certain conditions, e.g., ubiquitin ligase in a hypoxic state, such that the light-generating fusion protein is “turned on” only under such conditions.
Claims18
A transgenic non-human animal comprising a recombinant nucleic acid molecule stably integrated into the genome of said animal, said molecule encoding a light-generating fusion protein comprising a ligand binding site and a light-generating polypeptide moiety.
A transgenic non-human animal comprising a recombinant nucleic acid molecule stably integrated into the genome of said animal, said recombinant nucleic acid molecule comprising SEQ ID NO:25.
A method for the production of a transgenic non-human animal, comprising introduction of a recombinant nucleic acid molecule into a germ cell, an embryonic cell, an egg cell or a cell derived therefrom, said recombinant nucleic acid molecule encoding a light-generating fusion protein comprising a ligand binding site and a light-generating polypeptide moiety
Dependencies are inferred from the claim text.