Cloning bovines by nuclear transplantation
OwnerMark E. Westhusin, L. Garry Adams +3inventors
InventorsMark E. Westhusin, L. Garry Adams, Doris Hunter, Joe Wayne Templeton, Taeyoung Shin
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Abstract
Nuclear transfer methods and techniques involving cryopreserved bovine somatic cells, nuclei, or nuclear DNA, and the products thereof, are disclosed. These methods employ an enucleated oocyte as a recipient, which is fused with a donor nuclear genome from a cryopreserved bovine somatic cell to form a couplet. In addition, the a cloned bull exhibiting disease resistance is disclosed.
Claims67
A method of cloning a bovine comprising:
- (a) contacting an enucleated oocyte with a composition comprising nuclear DNA from a cryopreserved bovine somatic cell;
- (b) fusing the oocyte and the composition to form a cybrid;
- and
- (c) transferring the cybrid into the reproductive tract of a cow.
A bovine whose nuclear genome is identical to a single parent, wherein said single parent comprises a polymorphic NRAMP1 gene that confers disease resistance.
A bovine whose nuclear genome is identical to a single parent produced from a method comprising:
- (a) contacting an enucleated oocyte with a composition comprising nuclear DNA from a bovine somatic cell, wherein said bovine somatic cell is derived from a cell line comprising a polymorphic NRAMP1 gene;
- (b) fusing the oocyte and the composition to form a cybrid;
- and
- (c) transferring the cybrid into the reproductive tract of a cow.
A bovine whose nuclear genome is identical to a single ancestor and comprises a polymorphic NRAMP1 gene that confers disease resistance to brucellosis, tuberculosis, paratuberculosis or salmonellosis, wherein the bovine is produced from a method comprising:
- (a) contacting an enucleated oocyte with a composition comprising nuclear DNA from a bovine somatic cell, wherein said bovine somatic cell comprises the sequence of SEQ ID NO. 3;
- (b) fusing the oocyte and the composition to form a cybrid;
- and
- (c) transferring the cybrid into the reproductive tract of a cow.
Dependencies are inferred from the claim text.