Method for producing polyclonal and monoclonal antibodies
OwnerGeorg S. Wenglerinventor
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Abstract
This invention relates to a method for using an animal organism to produce monoclonal antibodies and/or human growth factors and/or for producing human immunoglobulins and blood components, and/or for producing polyclonal antiserums and/or for testing potential vaccines against infections. According to the invention, a human or animal stem cell is transplanted into a fertilised ovum of the animal organism to produce an intact human or animal haematopoiesis.
Claims26
A method for using an animal organism
- a. for producing monoclonal antibodies and/or
- b. for producing immunoglobulins and blood components and/or
- c. for producing polycgonal antibodies (antisera),
- wherein the step of transplanting a human or animal stem cell into an inseminated ovum of the animal organism is carried out for producing an intact human or animal hematopoiesis.
A method for producing human monoclonal antibodies (13), comprising the following steps,
- a. transplanting a human stem cell (1) of a specific human donor into an embryo (2) of a first animal organism (3);
- b. transplanting an animal stem cell (4) of a donor (10) and a human stem cell (1′) of the specific human donor into an embryo (5) of a second animal organism (6);
- c. injecting an antigen (7) for immunostimulating the first tolerant animal organism (14) born after transplantation according to step (a);
- d. isolating human antibody-producing B cells (8) from the first tolerant organism (14);
- e. fusing the B cells (8) with tumor cells (9), preferably myeloma cells, of an organism (10) donating the stem cells (4) according to step (b), for producing hybridoma cells;
- f. injecting the hybridoma cells (11) into the second double-tolerant animal organism (12) born after transplantation according to step b.;
- g. isolating the monoclonal human antibodies (13) from the resulting ascites (15), the resulting blood serum, the produced milk or the laid eggs of the second double-tolerant animal organism (12).
A method for producing mouse-monoclonal antibodies, comprising the following steps:
- a. transplanting a stem cell (FLZ) of a mouse donor animal (M) into an embryo (E) of an animal organism (T);
- b. injecting hybridoma cells (HC) of the mouse-donor animal (M) into the mouse-tolerant animal organism (C) born after transplantation according to step a.;
- and
- c. isolating mouse-monoclonal antibodies (mAb) from the resulting ascites (ASC), the resulting blood serum, the produced milk or the laid eggs of the mouse-tolerant animal (C).
A bioreactor for producing monoclonal antibodies, comprising
- at least one part of a life-sustained animal body having a functioning blood circulation;
- and
- at least one functioning organ of the animal body in which after injection of antibody-producing hybridoma cells of a donor monoclonal antibodies can be produced or after injection of antigens antisera can be produced or are producible in the blood products,
- wherein the animal body is tolerant to the donor of the cells.
Dependencies are inferred from the claim text.