Method for quantifying nucleic acid by cell counting
OwnerYukio Sudo, Masato Someinventors
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Abstract
The present invention provides a method for quantifying the amount of nucleic acid existing in a specimen which comprises steps of: (1) quantitatively measuring the amount of nucleic acid in the specimen; (2) measuring the distribution of cell types existing in the specimen; and (3) correcting the measured value of the amount of nucleic acid based on the measured value of cell distribution. According to the present invention, the expression level of genes in the target cell can be measured without isolating target cells from control cells in the sample comprising one or more types of control cells and one type of target cell.
Claims12
A method for quantifying the amount of nucleic acid existing in a specimen which comprises steps of:
- (1) quantitatively measuring the amount of nucleic acid in the specimen;
- (2) measuring the distribution of cell types existing in the specimen;
- and
- (3) correcting the measured value of the amount of nucleic acid based on the measured value of cell distribution.
A method for diagnosing disease states by measuring the amount of mRNA being expressed in a specimen, which comprises steps of:
- (1) quantitatively measuring the amount of mRNA in the specimen;
- (2) measuring the distribution of cell types existing in the specimen;
- and
- (3) correcting the measured value of the mRNA based on the measured value of cell distribution.
Dependencies are inferred from the claim text.