Methods and compositions comprising Renilla GFP
AssigneeRigel Pharmaceuticals, Inc.
InventorsDavid Anderson, Beau Peelle
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Abstract
The invention relates to methods and compositions utilizing Renilla green fluorescent proteins (rGFP), and Ptilosarcus green fluorescent proteins (pGFP). In particular, the invention relates to the use of Renilla GFP or Ptilosarcus GFP proteins as reporters for cell assays, particularly intracellular assays, including methods of screening libraries using rGFP or pGFP.
Claims46
A retroviral vector comprising a fusion nucleic acid comprising:
- a) a promoter;
- and
- b) Renilla GFP gene.
A retroviral vector comprising a fusion nucleic acid comprising:
- a) a promoter;
- and
- b) Ptilosarcus GFP gene.
A nucleic acid vector comprising a fusion nucleic acid comprising:
- a) a promoter;
- b) Renilla GFP gene;
- c) a separation site;
- and
- d) a gene of interest.
A nucleic acid vector comprising a fusion nucleic acid comprising:
- a) a promoter;
- b) Ptilosarcus GFP gene;
- c) a separation site;
- and
- d) a gene of interest.
A fusion nucleic acid comprising:
- a) a gene of interest;
- and
- b) a gene encoding codon optimized Renilla muelleri GFP
A fusion nucleic acid comprising:
- a) a gene of interest;
- and
- b) a gene encoding codon optimized Ptilosarcus GFP
A method of screening for bioactive agents, said method comprising:
- a) combining a candidate bioactive agent and a cell comprising a fusion nucleic acid comprising
- i) a promoter
- ii) a codon optimized Renilla muelleri GFP;
- b) screening said cells for an altered phenotype.
A method of screening for bioactive agents, said method comprising
- a) combining a candidate bioactive agent and a cell comprising a fusion nucleic acid comprising
- i) a promoter
- ii) a codon optimized Ptilosarcus GFP;
- b) screening said cells for an altered phenotype.
Dependencies are inferred from the claim text.