Macromolecular conjugates and processes for preparing the same
OwnerJohn C. Russellinventor
Life of this patent
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Abstract
Making a suspended or soluble macromolecular conjugate comprising binding a first macromolecule to a solid via a stable, disruptable bond, stably linking additional macromolecules, and releasing the macromolecular conjugate, as well as macromolecular conjugates prepared by the method.
Claims29
A method of making a suspended or soluble protein conjugate, the method comprising the steps of:
- a) providing a reactive surface, protein that is capable of forming a stable, disruptable bond with the reactive solid-surface, and a Second Macromolecule,
- b) contacting the protein to the reactive solid-surface to form a surface-bound protein complex,
- c) if necessary, activating the protein, or Second Macromolecule, or both,
- d) contacting the surface bound protein with a reactive Second Macromolecule to form a solid-surface:protein:
- Second Macromolecule stable complex,
- e) disrupting the stable bond between the solid-surface and the protein conjugate in a liquid medium to yield a suspended or soluble protein conjugate comprising the protein and the Second Macromolecule.
A method of making a suspended or soluble Macromolecular Conjugate, the method comprising the steps of:
- a) providing a reactive surface,
- b) providing a First Macromolecule that is capable of forming a stable, disruptable bond with the reactive solid-surface,
- c) contacting the First Macromolecule to the reactive solid-surface to form a surface-bound First Macromolecule complex,
- d) if necessary, activating the First Macromolecule, or Second Macromolecule, or both,
- e) contacting the surface bound First Macromolecule with a reactive Second Macromolecule to form a solid-surface:First Macromolecule:Second Macromolecule stable complex,
- f) disrupting the stable bond between the solid-surface and the First Macromolecule conjugate in a liquid medium to yield a suspended or soluble Macromolecular Conjugate conjugate comprising the First Macromolecule and the Second Macromolecule,
- wherein the First Macromolecule comprises a plurality of reactive moieties capable of interacting with the Second Macromolecule, and the Second Macromolecule comprises a plurality of reactive moieties capable of interacting with the First Macromolecule.
The method of claim 15, wherein the capping compound is zwitterionic.
A composition comprising a population of conjugates, wherein each conjugate of the population comprises a single antibody.
A composition comprising a population of conjugates, wherein each conjugate of the population comprises a predetermined number of antibodies and the number of antibodies is between 2 and 30.
A conjugate comprising three layers of macromolecules, wherein at least two of the three layers comprise a plurality of macromolecules, and wherein the layers form a surface in three dimensions.
A conjugate comprising
- a specific binding member,
- a plurality of end-point molecules, and
- a plurality of spacer molecules, wherein
- the spacer molecules substantially separate the end-point molecules.
A population of conjugates, wherein
- substantially each conjugate of the molecule comprises from 1 to 30 molecules of a specific binding members,
- each of the specific binding members is disposed on the surface of the conjugate, and substantially each conjugate comprises a core, wherein the core comprises at least one molecule that is not disposed on the surface of the conjugate.
A kit comprising a reactive conjugate complex, and a cleavage reagent wherein,
- the reactive conjugate complex comprises a solid bonded with a First Macromolecule covalently complexed with a Reactive Macromolecule, and
- the cleavage reagent is capable of cleaving the bond between the solid and the First Macromolecule.
Dependencies are inferred from the claim text.