Targeting peptides

A pharmaceutical composition comprising a targeting peptide in association with a vehicle, the targeting peptide comprising an amino acid sequence selected from the group consisting of:

• AASARLP, VYFPAPN, FSMSTPS, IVAQPRL, FPQTYTT, NIAAFSL, QPRLLHH, NIIPAPT, SPTYPRR, TRSQPPL, NTGPNRV, PPPDWTF, SHFSHLR, AFNYPPH, DFLQVSP, SPDHLFC, LEHPPTT, TYPSSEW, IPMHLHN, TSESPTV, YSLSRSL, NHLSALY, TYSLKSA, TSTMPSR, ETIKTNT, ATGFATP, TNSQPSP, TSFFMPP, TAAYRFW, LPPSLYS, SPSVVPF, HSLTFSI, WNSTTQA, HFTHPTH, AGATAMS, STYPIIR, SWNHARV, NHWHGGL, GILSPSH, EAVPTYS, INSNAPG, YSTHSTR, SDLATVR, INSVSPH, MSSPGPA, LPTKTLF, AAWPTSS, LTAELTP, KIDGTPR, VEPARAS, SIGYPLP, WTSDELH, TLGLHMS, LSNFHSS, SLPRNSD, GYQQVFQ, MSPPGPA, LCMTTLV, SEVAVQG, MAMPQPADHNNS, VSGMSVPVQLAR, MTQTPRTTPWPD, MSLTTPPAVARP, MSNNPIRPPTSG, MTQVYTPPPTST, MTGSQQTLHPPP, MATQPLSGSRLSG, MNMTPPPHSPPK, MTPFPTSNEANL, AMSMTTMPHSPN, MSDLLIEYPPYI, MTLPHELRDGAL, AAVPPPYVMSRP, MSQTPYARPQYV, MTSNPHLNPGPR, MGHNINIPRTPL, LSTPLPYDMRRS, MTRIQDSPYDLR, MSTPPIREQAAH, MTNLPTVTQFPP, MTPIATSIPPQM, MTPTTPIPSLPQ, MTSPHPQTPNLT, MTQQPPLPHPAK, LAKPLPTTSNTG, LSKPIPHIPSSIG, CICRGVGCCLLL, LQPPSMITHPST, LTPPNQVLNPLY, AFPMVGGPDHFR, MLMPQPAHHNNS, AQAMANPLGSHI, SSRIPGFPDPLH, SMRGLPELNPRI, MSSPTVSSAPQY, VLSMQTPPTPLL, THAMSHLDKAH, MAVQPPNTSTSN, MAINDTYPPPRP, MMPPPTSLPSPS, LAQNPIYRAHPH, MQPRPQTLTPAS, LTVPVPVSFAVH, LTSPFSTPLNPR, MAGQPKDSSKTL, ANTPPHTILSTE, MGMTVPENLIVQ, and MTPIQSTQYPHS, or a derivative thereof, the vehicle carrying a pharmaceutically active agent, and a pharmaceutically acceptable carrier.

A method of targeting a material to cell, said method comprising bringing into association a targeting peptide comprising amino acid sequence selected from the group consisting of:

• AASARLP, VYFPAPN, FSMSTPS, IVAQPRL, FPQTYTT, NIAAFSL, QPRLLHH, NIIPAPT, SPTYPRR, TRSQPPL, NTGPNRV, PPPDWTF, SHFSHLR, AFNYPPH, DFLQVSP, SPDHLFC, LEHPPTT, TYPSSEW, IPMHLHN, TSESPTV, YSLSRSL, NHLSALY, TYSLKSA, TSTMPSR, ETIKTNT, ATGFATP, TNSQPSP, TSFFMPP, TAAYRFW, LPPSLYS, SPSVVPF, HSLTFSI, WNSTTQA, HFTHPTH, AGATAMS, STYPIIR, SWNHARV, NHWHGGL, GILSPSH, EAVPTYS, INSNAPG, YSTHSTR, SDLATVR, INSVSPH, MSSPGPA, LPTKTLF, AAWPTSS, LTAELTP, KIDGTPR, VEPARAS, SIGYPLP, WTSDELH, TLGLHMS, LSNFHSS, SLPRNSD, GYQQVFQ, MSPPGPA, LCMTTLV, SEVAVQG, MAMPQPADHNNS, VSGMSVPVQLAR, MTQTPRTTPWPD, MSLTTPPAVARP, MSNNPIRPPTSG, MTQVYTPPPTST, MTGSQQTLHPPP, MATQPLSGSRLSG, MNMTPPPHSPPK, MTPFPTSNEANL, AMSMTTMPHSPN, MSDLLIEYPPYI, MTLPHELRDGAL, AAVPPPYVMSRP, MSQTPYARPQYV, MTSNPHLNPGPR, MGHNINIPRTPL, LSTPLPYDMRRS, MTRIQDSPYDLR, MSTPPIREQAAH, MTNLPTVTQFPP, MTPIATSIPPQM, MTPTTPIPSLPQ, MTSPHPQTPNLT, MTQQPPLPHPAK, LAKPLPTTSNTG, LSKPIPHIPSSIG, CICRGVGCCLLL, LQPPSMITHPST, LTPPNQVLNPLY, AFPMVGGPDHFR, MLMPQPAHHNNS, AQAMANPLGSHI, SSRIPGFPDPLH, SMRGLPELNPRI, MSSPTVSSAPQY, VLSMQTPPTPLL, THAMSHLDKAH, MAVQPPNTSTSN, MAINDTYPPPRP, MMPPPTSLPSPS, LAQNPIYRAHPH, MQPRPQTLTPAS, LTVPVPVSFAVH, LTSPFSTPLNPR, MAGQPKDSSKTL, ANTPPHTILSTE, MGMTVPENLIVQ, and MTPIQSTQYPHS, or a derivative thereof, with the material to be targeted to form a complex and exposing the complex to a cell(s).

A kit comprising a targeting peptide comprising an amino acid sequence selected from the group consisting of:

• AASARLP, VYFPAPN, FSMSTPS, IVAQPRL, FPQTYTT, NIAAFSL, QPRLLHH, NIIPAPT, SPTYPRR, TRSQPPL, NTGPNRV, PPPDWTF, SHFSHLR, AFNYPPH, DFLQVSP, SPDHLFC, LEHPPTT, TYPSSEW, IPMHLHN, TSESPTV, YSLSRSL, NHLSALY, TYSLKSA, TSTMPSR, ETIKTNT, ATGFATP, TNSQPSP, TSFFMPP, TAAYRFW, LPPSLYS, SPSVVPF, HSLTFSI, WNSTTQA, HFTHPTH, AGATAMS, STYPIIR, SWNHARV, NHWHGGL, GILSPSH, EAVPTYS, INSNAPG, YSTHSTR, SDLATVR, INSVSPH, MSSPGPA, LPTKTLF, AAWPTSS, LTAELTP, KIDGTPR, VEPARAS, SIGYPLP, WTSDELH, TLGLHMS, LSNFHSS, SLPRNSD, GYQQVFQ, MSPPGPA, LCMTTLV, SEVAVQG, MAMPQPADHNNS, VSGMSVPVQLAR, MTQTPRTTPWPD, MSLTTPPAVARP, MSNNPIRPPTSG, MTQVYTPPPTST, MTGSQQTLHPPP, MATQPLSGSRLSG, MNMTPPPHSPPK, MTPFPTSNEANL, AMSMTTMPHSPN, MSDLLIEYPPYI, MTLPHELRDGAL, AAVPPPYVMSRP, MSQTPYARPQYV, MTSNPHLNPGPR, MGHNINIPRTPL, LSTPLPYDMRRS, MTRIQDSPYDLR, MSTPPIREQAAH, MTNLPTVTQFPP, MTPIATSIPPQM, MTPTTPIPSLPQ, MTSPHPQTPNLT, MTQQPPLPHPAK, LAKPLPTTSNTG, LSKPIPHIPSSIG, CICRGVGCCLLL, LQPPSMITHPST, LTPPNQVLNPLY, AFPMVGGPDHFR, MLMPQPAHHNNS, AQAMANPLGSHI, SSRIPGFPDPLH, SMRGLPELNPRI, MSSPTVSSAPQY, VLSMQTPPTPLL, THAMSHLDKAH, MAVQPPNTSTSN, MAINDTYPPPRP, MMPPPTSLPSPS, LAQNPIYRAHPH, MQPRPQTLTPAS, LTVPVPVSFAVH, LTSPFSTPLNPR, MAGQPKDSSKTL, ANTPPHTILSTE, MGMTVPENLIVQ, and MTPIQSTQYPHS, or a derivative thereof, for transfecting or identifying cell types in vitro.

A method for screening targeting peptides capable of binding to an endothelial cell, said method comprising:

• inserting a polynucleotide encoding a potential endothelial cell-binding peptide into an expression vector;
• expressing the peptide;
• conducting a pre-screening step with the expressed peptides using non-endothelial cells in order to select for the expressed peptides with reduced or negligible binding to the non-endothelial cells;
• further screening the expressed peptides which exhibited reduced or negligible binding to the non-endothelial cells using endothelial cells;
• and
• selecting for the expressed peptides which display selective and efficient binding to the endothelial cells.

A method of treating a disease, said method comprising administering a pharmaceutical agent in association with a targeting peptide, said targeting peptide comprising amino acid sequence selected from the group consisting of:

• AASARLP, VYFPAPN, FSMSTPS, IVAQPRL, FPQTYTT, NIAAFSL, QPRLLHH, NIIPAPT, SPTYPRR, TRSQPPL, NTGPNRV, PPPDWTF, SHFSHLR, AFNYPPH, DFLQVSP, SPDHLFC, LEHPPTT, TYPSSEW, IPMHLHN, TSESPTV, YSLSRSL, NHLSALY, TYSLKSA, TSTMPSR, ETIKTNT, ATGFATP, TNSQPSP, TSFFMPP, TAAYRFW, LPPSLYS, SPSVVPF, HSLTFSI, WNSTTQA, HFTHPTH, AGATAMS, STYPIIR, SWNHARV, NHWHGGL, GILSPSH, EAVPTYS, INSNAPG, YSTHSTR, SDLATVR, INSVSPH, MSSPGPA, LPTKTLF, AAWPTSS, LTAELTP, KIDGTPR, VEPARAS, SIGYPLP, WTSDELH, TLGLHMS, LSNFHSS, SLPRNSD, GYQQVFQ, MSPPGPA, LCMTTLV, SEVAVQG, MAMPQPADHNNS, VSGMSVPVQLAR, MTQTPRTTPWPD, MSLTTPPAVARP, MSNNPIRPPTSG, MTQVYTPPPTST, MTGSQQTLHPPP, MATQPLSGSRLSG, MNMTPPPHSPPK, MTPFPTSNEANL, AMSMTTMPHSPN, MSDLLIEYPPYI, MTLPHELRDGAL, AAVPPPYVMSRP, MSQTPYARPQYV, MTSNPHLNPGPR, MGHNINIPRTPL, LSTPLPYDMRRS, MTRIQDSPYDLR, MSTPPIREQAAH, MTNLPTVTQFPP, MTPIATSIPPQM, MTPTTPIPSLPQ, MTSPHPQTPNLT, MTQQPPLPHPAK, LAKPLPTTSNTG, LSKPIPHIPSSIG, CICRGVGCCLLL, LQPPSMITHPST, LTPPNQVLNPLY, AFPMVGGPDHFR, MLMPQPAHHNNS, AQAMANPLGSHI, SSRIPGFPDPLH, SMRGLPELNPRI, MSSPTVSSAPQY, VLSMQTPPTPLL, THAMSHLDKAH, MAVQPPNTSTSN, MAINDTYPPPRP, MMPPPTSLPSPS, LAQNPIYRAHPH, MQPRPQTLTPAS, LTVPVPVSFAVH, LTSPFSTPLNPR, MAGQPKDSSKTL, ANTPPHTILSTE, MGMTVPENLIVQ, and MTPIQSTQYPHS, or a derivative thereof, wherein said targeting peptide delivers the pharmaceutical agent for uptake by a target cell.