Crosslinked enzyme aggregates and crosslinking agent therefor
AssigneeAvantium International B.V.
InventorsLinqiu Cao, Jeoffrey Elzinga
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Abstract
Described is a method for the preparation of crosslinked enzyme aggregates, comprising the steps of: A—providing a plurality of enzyme molecules, B—aggregating the enzymes in a liquid medium, comprising a precipitating agent, C—crosslinking the aggregated enzymes to one another by providing a crosslinking agent in the liquid medium, wherein the crosslinking agent being prepared by combining a first and a second compound each having at least two reactive groups, the reactive groups of the first compound being primary amino groups, the reactive groups of the second compound being aldehyde groups.
Claims13
Method for the preparation of crosslinked enzyme aggregates, comprising the steps of:
- A—providing a plurality of enzyme molecules,
- B—aggregating the enzymes in a liquid medium, comprising a precipitating agent,
- C—crosslinking the aggregated enzymes to one another by providing a crosslinking agent in the liquid medium,
- wherein the crosslinking agent being prepared by combining a first and a second compound each having at least two reactive groups, the reactive groups of the first compound being primary amino groups, the reactive groups of the second compound being aldehyde groups.
Crosslinking agent prepared by combining a first compound comprising at least two carbon atoms, the termini of the backbone being defined as α and ω, respectively, the said termini both comprising the active groups, the second compound being a dialdehyde.
Dependencies are inferred from the claim text.