Traversal of nucleic acid molecules through a fluid space and expression in repair cells
AssigneeSelective Genetics, Inc.
InventorsBarbara A. Sosnowski, Glenn Pierce
Life of this patent
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Abstract
Disclosed are methods for use in transferring nucleic acids into cells at a wound site associated with a fluid space. These gene transfer protocols are suitable for use in transferring various nucleic acids into cartilage, cardiac muscle, and other tissues, and have many uses including treating diseases such as arthritis and ischemic heart disease, and promoting wound healing. The invention further disclosed pharmaceutical compositions that may be used in the practice of the invention to transfer the nucleic acid of interest. Such compositions include any multi-partitioned biocompatible matrix in combination with multiple nucleic acids of interest.
Claims87
A method for transferring a nucleic acid molecule into cells associated with a fluid space, comprising contacting a wound site with a composition comprising a nucleic acid molecule and a biocompatible matrix, the wound site being situated in a tissue associated with the fluid space.
A method of stimulating gene expression in cartilage progenitor cells located within a cartilage progenitor tissue site of an animal, comprising contacting the tissue site with a composition comprising a chondrogenic gene and a biocompatible matrix.
The method of 44 wherein the chondrogenic gene is a gene selective from the group consisting of BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, BMP8, BMP9, BMP10, BMP11, BMP12, BMP13 genes.
A method of stimulating cartilage repair or regeneration comprising implanting at a cartilage defective site a matrix-gene composition comprising a chondrogenic gene and a biocompatible matrix.
A method of treating arthritis comprising implanting at a cartilage defective site a matrix-gene composition comprising a chondrogenic gene and a biocompatible matrix.
A method of treating ischemic heart disease comprising implanting a matrix-gene composition comprising an angiogenic gene and a biocompatible matrix into an ischemic region.
A composition comprising multiple genes associated with a multi-partitioned biocompatible matrix.
Dependencies are inferred from the claim text.