US 2003/0148978 A1 · Oligonucleotides antisense to mouse pituitary tumor transforming gene carboxy-terminal (PTTG-C) and methods of use thereof to inhibit neoplastic cellular proliferation and/or transformation

Abstract

Disclosed is a method of inhibiting neoplastic cellular proliferation and/or transformation of mammalian cells, including cells of human origin, in vitro or in vivo. The inventive method involves the delivery of polynucleotides antisense to PTTG-C-related polynucleotides to mammalian cells, whether in vitro or in vivo, to inhibit the endogenous expression of PTTG. Also disclosed are compositions comprising antisense PTTG carboxy-terminal-related polynucleotides, including compositions comprising expression vectors containing the PTTG-C-related polynucleotides. Kits comprising the inventive compositions are also disclosed for the treatment of neoplastic cellular proliferation in vitro or in vivo

Claims17

Dependent claims

A method of inhibiting neoplastic cellular proliferation and/or transformation of a mammalian cell comprising:

delivering to a mammalian cell that overexpresses PTTG, a composition comprising a PTTG carboxy-terminal-related polynucleotide that is antisense to:
(A) (SEQ ID NO:19);
(B) a degenerate coding sequence of (A);
or
(C) a polynucleotide fragment of (A) or (B) that comprises a coding sequence that encodes a proline-rich region;
said PTTG carboxy-terminal-related polynucleotide being complexed with a cellular uptake-enhancing agent, in an amount and under conditions sufficient to allow the polynucleotide to enter the cell, whereby expression of PTTG protein is prevented and neoplastic cellular proliferation and/or transformation of the cell is inhibited.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/262,264Filed 30 Sep 2002

Priority

21 Nov 1996

Published

7 Aug 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

A01K 67/027
Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals: new or modified breeds of vertebrates
A61K 48/00
Medicinal preparations containing genetic material which is inserted into cells of the
C07K 14/47
From vertebrates: from mammals
C07K 14/82
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: translation products from oncogenes

Family

Application60/031,338Us provisional application · filed 21 Nov 1996

Application60/065,825Us provisional application · filed 14 Nov 1997

Application08/894,251Continuation in part · filed 23 Jul 1999

Application08/894,251A 371 of international · filed 23 Jul 1999

Application09/569,956Division · filed 12 May 2000

Application09/569,956Continuation in part · filed 12 May 2000

Application10/262,264Division · filed 30 Sep 2002

US 6,455,305 B1Related patent

US 6,894,031 B1Related patent

US 2003/0148978 A1This patent

From the US filing history. Foreign family members aren’t listed.

Oligonucleotides antisense to mouse pituitary tumor transforming gene carboxy-terminal (PTTG-C) and methods of use thereof to inhibit neoplastic cellular proliferation and/or transformation

Life of this patent

Oligonucleotides antisense to mouse pituitary tumor transforming gene carboxy-terminal (PTTG-C) and methods of use thereof to inhibit neoplastic cellular proliferation and/or transformation

Life of this patent

Abstract

Claims17