Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
AssigneeAdvanced Research and Technology Institute, Inc.
InventorsShuming Nie, Xiaohu Gao, Mingyong Han
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Abstract
The present invention provides a method of preparing a multicolor quantum dot-tagged bead, a multicolor quantum dot-tagged bead, a conjugate thereof, and a composition comprising such a bead or conjugate. Additionally, the present invention provides a method of making a conjugate thereof and methods of using a conjugate for multiplexed analysis of target molecules.
Claims65
A method of preparing a multicolor quantum dot-tagged bead, which method comprises:
- (a) providing at least one porous polymer bead, wherein the pores of the bead are large enough to incorporate quantum dots;
- (b) combining predetermined amounts of multicolor quantum dots with at least one bead;
- and
- (c) isolating the multicolor quantum dot-tagged bead.
A multicolor quantum dot-tagged bead, which comprises at least one quantum dot and a porous polymer bead, wherein the bead has pores large enough to incorporate the quantum dot, and wherein the quantum dots are present in a precisely controlled ratio.
A conjugate comprising at least one quantum dot and a porous polymer bead, and a probe, wherein the bead has pores large enough to incorporate the quantum dot, wherein the quantum dots are present in a precisely controlled ratio, wherein the conjugate hybridizes to a target sequence, wherein the probe is attached to the bead, and wherein the target is labeled with a quantum dot.
A method of preparing a multicolor quantum dot-tagged bead, which method comprises:
- (a) providing at least one porous bead by solvent-system polymerization that includes about 0.3-5% by volume of a cross-linking agent, wherein the pores of the bead have an average diameter of at least about 1 nm;
- (b) combining predetermined amounts of multicolor quantum dots with at least one bead;
- and
- (c) isolating the multicolor quantum dot-tagged bead.
Dependencies are inferred from the claim text.