Cell-specific adenovirus vectors comprising an internal ribosome entry site
AssigneeCell Genesys, Inc.
InventorsDe-Chao Yu, Yuanhao Li, Andrew S. Little, Daniel R. Henderson
Life of this patent
Estimating the term…
Abstract
Disclosed herein are replication-competent adenovirus vectors comprising co-transcribed first and second genes under transcriptional control of a heterologous, target cell-specific transcriptional regulatory element (TRE), wherein the second gene is under translational control of an internal ribosome entry site. Methods for the preparation and use of such vectors are also provided. The vectors provide target cell-specific virus replication in applications such as cancer therapy and gene therapy.
Claims58
A replication-competent adenovirus vector comprising first and second genes co-transcribed as a single mRNA wherein the first and the second genes are under transcriptional control of a heterologous, target cell-specific transcriptional regulatory element (TRE), wherein the second gene has a mutation in or deletion of its endogenous promoter and is under translational control of an internal ribosome entry site (IRES) and wherein said vector exhibits greater specificity for the target cell than an adenovirus vector comprising a target cell-specific TRE operably linked to a gene and lacking an IRES.
An adenovirus vector comprising a gene under transcriptional control of a melanocyte-specific TRE.
An adenovirus vector comprising E1B under transcriptional control of a heterologous, target cell specific TRE, wherein E1B has a deletion of part or all of the 19-kDa region.
Dependencies are inferred from the claim text.