Preparation and compositions for antrodia camphorata mycelium biologically active material

Process to prepare the active material from Antrodia camphorata mycelium, which includes the following procedures:

• (1) Plate Culture:
• Seed mycelium on plate and maintain at 30° C. for two weeks.
• (2) Beaker Culture:
• collect fungus grown on plate to put in beaker. Use the following culture medium at about 30° C. and pH 4.5 with vibrator operation at 50-250 rpm until initial log period, i.e. About 5-7 days;
• 11 Culture Medium Formula Components Content (weight %) Cereals (like flour) 1 Egg white 0.1 Magnesium Sulfate 0.05 Potassium hydrogenphosphate 0.05 Ferric Sulfate 0.05 Sucrose 2 Enzyme Extract, Powder, paste 0.5 Beans (like soy bean powder, green bean powder 0.2 etc.)
• (3) Fermentation Tank Culture:
• Transfer the cultured species to fermentation tank containing culture medium (same as that in beaker). Perform culturing for about 10 days at 30° C., a tank pressure of 0.5-1.0 kg/cm2, pH below 4.5, with input of air at 150 liter/minute, under agitation at 200 rpm to obtain suspension culture solution for Antrodia camphorata mycelium containing mycelium and supernatant;
• (4) Centrifuge the solution from step (3) to separate out mycelium and supernatant;
• (5) Use solvents to extract the biologically active material.