Novel mammalian multipotent neural stem cells and compositions, methods of preparation and methods of administration thereof
AssigneeThe Board of Trustees of the University of Illinois
InventorsKiminobu Sugaya, Tingyu Qu, Jose S. Pulido
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Abstract
This invention relates to novel mammalian multipotent neural stem cells (MNSCs), compositions thereof, and methods of preparing and administering the cells to diseased, aged or damaged tissue such that the cells properly migrate and differentiate and a neurological or corporal deficit is improved or remedied as a result.
Claims48
A method of preparing one or a plurality of mammalian multipotent stem cells that can forms a cluster comprising the steps of
- a. isolating mammalian neural stem cells derived from mammalian neural tissue containing at least one neural stem cell,
- b. culturing said cells in a cell culture media under conditions that permit proliferation of the neural stem cells in vitro,
- c. harvesting the one or plurality of mammalian multipotent stem cells that can form a cluster produced in (b),
- wherein said one or a plurality of mammalian multipotent stem cells that can form a cluster is adapted for proliferation, migration and differentiation in mammalian tissue when introduced thereto.
A method of making a retinal cell, the method comprising contacting a neural stem cell before or during differentiation with an effective amount of growth factor selected from the group consisting of TGF-b3, IGF-1 and CNTF, for an effective period, wherein the growth factor-contacted neural stem cells become capable of differentiating into retinal cells.
Dependencies are inferred from the claim text.