Methods of inducing differentiation in stem cells, methods of generating tissue using scaffold matrices derived from micro-organs and stem cells, methods of producing adult stem cells and methods of continuously generating stem cells by implantation of micro-organs as sources of stem cells

A method of inducing stem cells differentiation, the method comprising co-culturing isolated stem cells and at least one micro-organ, thereby inducing stem cells differentiation.

A method of inducing stem cells differentiation, the method comprising culturing isolated stem cells in micro-organ conditioned medium, thereby inducing stem cells differentiation.

A method of generating an artificial micro-organ comprising:

• (a) providing an acellular three dimensional scaffold, said acellular three dimensional scaffold being of dimensions selected such that when populated with cells, said cells positioned deepest within said scaffold are at least about 100 micrometers and not more than about 225 micrometers away from said cells positioned at a nearest surface formed on said scaffold;
• and
• (b) seeding said acellular three dimensional scaffold with cells at least until said cells repopulate said acellular three dimensional scaffold.

A method of isolating adult stem cells comprising culturing at least one micro-organ derived from an adult tissue in a culture and isolating cells migrating out of said at least one micro-organ, said cells being adult stem cells.