Transcription factor

A DNA construct comprising a DNA sequence encoding a transcription factor exhibiting activity in regulating the expression of an alpha-amylase promoter in a filamentous fungus, which DNA sequence comprises

• a) the transcription factor encoding part of the DNA sequence cloned into plasmid pToC320 present in E. coli ToC1058, DSM 10666, or
• b) an analogue of the DNA sequence defined in a), which
• i) is at least 60% homologous with the DNA sequence defined in a), or
• ii) hybridizes with the same nucleotide probe as the DNA sequence defined in a), or
• iii) encodes a transcription factor which is at least 50% homologous with the transcription factor encoded by a DNA sequence comprising the DNA sequence defined in a), or
• iv) encodes a transcription factor which is immunologically reactive with an antibody raised against the purified transcription factor encoded by the DNA sequence defined in a), or
• v) complements the mutation in DSM 10671, i.e. makes DSM 10671 able to grow on cyclodextrin and produce lipase when transformed with said DNA sequence.

A DNA construct comprising a DNA sequence encoding a transcription factor which exhibits activity in regulating the expression of a promoter in a filamentous fungus, wherein the DNA sequence is any of:

• (a) a DNA sequence that is at least 70% homologous with a sequence of nucleotides 1691-2676, 2743-3193 and 3278-3652 of SEQ ID NOS:
• 1 and 2, wherein the homology is determined using GAP with a GAP creation penalty of 5.0 and a GAP extension penalty of 0.3;
• (b) a DNA sequence that hybridizes with a sequence of nucleotides 1691-2676, 2743-3193 and 3278-3652 of SEQ ID NOS:
• 1 and 2 under the following hybridization conditions, prehybridization and in a solution of 5×SSC, 5×Denhardt's solution, 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA at 65° C., followed by washing two times for 30 minutes in 2×SSC and 0.5% SDS at a temperature of 65° C. or 70° C.;
• (c) a DNA sequence that encodes a transcription factor having an amino acid sequence which is at least 90% homologous with SEQ ID NO:
• 3, wherein the homology is determined using GAP with a GAP creation penalty of 3.0 and a GAP extension penalty of 0.1;
• (d) a DNA sequence encoding a transcription factor having an amino acid sequence which comprises a Gal 4-type zinc finger sequence of amino acids 28-54 of SEQ ID NO:
• 3;
• and
• (e) a DNA sequence encoding a fragment of SEQ ID NO:
• 3 which exhibits activity in regulating the expression of a promoter in a filamentous fungus.