Media and methods for cultivation of microorganisms
AssigneeNorth Carolina State University
InventorsEdward B. Breitschwerdt, Sushama Sontakke
Life of this patent
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Abstract
The present invention provides culture media and methods for culturing organisms, preferably microorganisms, more preferably fastidious microorganisms. Also provided are methods of isolating and detecting organisms using the inventive culture media.
Claims49
A culture medium for growing a fastidious microorganism comprising sufficient biosynthetic building blocks and other organic molecules to support the growth of the fastidious microorganism in culture.
A method of culturing a fastidious microorganism comprising culturing a sample containing a fastidious microorganism in a culture medium comprising sufficient biosynthetic building blocks and other organic molecules to support the growth of a fastidious microorganism in culture for a time and under conditions sufficient to grow a fastidious microorganism present in the sample.
A method of detecting a fastidious microorganism in a sample comprising:
- culturing a sample containing a fastidious microorganism in a culture medium comprising sufficient biosynthetic building blocks and other organic molecules to support the growth of a fastidious microorganism in culture for a time and under conditions sufficient to grow the fastidious microorganism;
- and
- detecting the fastidious microorganism.
A method of identifying a fastidious microorganism in a sample, comprising:
- culturing a sample containing a fastidious microorganism in a culture medium comprising sufficient biosynthetic building blocks and other organic molecules to support the growth of a fastidious microorganism in culture for a time and under conditions sufficient to grow the fastidious microorganism;
- and
- identifying the fastidious microorganism.
A method of identifying a compound that binds to a fastidious microorganism, comprising:
- culturing a sample containing a fastidious microorganism in a culture medium comprising sufficient biosynthetic building blocks to support the growth of a fastidious microorganism in culture for a time and under conditions sufficient to grow the fastidious microorganism;
- contacting the fastidious microorganism with a compound;
- and
- detecting binding between the fastidious microorganism and the compound.
A method of diagnosing a mammalian subject with an infection by a fastidious microorganism, comprising:
- culturing a sample from a mammalian subject containing a fastidious microorganism in a culture medium comprising sufficient biosynthetic building blocks and other organic molecules to support the growth of a fastidious microorganism in culture for a time and under conditions sufficient to grow the fastidious microorganism;
- identifying the fastidious microorganism in the cultured sample;
- and
- diagnosing the subject as having an infection with the fastidious microorganism.
A method of diagnosing a disorder in a subject comprising:
- culturing a sample containing a fastidious microorganism in a culture medium comprising sufficient biosynthetic building blocks and other organic molecules to support the growth of a fastidious microorganism in culture for a time and under conditions sufficient to grow the fastidious microorganism;
- and
- identifying the fastidious microorganism in the cultured sample;
- wherein the presence of the fastidious microorganism in the sample is associated with the disorder.
A method of culturing a nanobacterium comprising culturing a sample comprising the nanobacterium in a culture medium comprising sufficient biosynthetic building blocks and other organic molecules to support the growth of nanobacterium in culture for a time and under conditions sufficient to grow the nanobacterium.
A method of culturing Corynebacteria, comprising culturing a sample containing Corynebacteria in a culture medium comprising sufficient biosynthetic building blocks and other organic molecules to support the growth of Corynebacteria in culture for a time and under conditions sufficient to grow Corynebacteria present in the sample.
Dependencies are inferred from the claim text.