Sialidase and recombinant cell lines
AssigneeGenentech, Inc.
InventorsThomas G. Warner, Mary B. Sliwkowski
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Abstract
A recombinant cell line has a constitutive sialidase whose functional expression is disrupted, for example by homologous recombination or using antisense RNA. Sialidase is purified from cell culture fluid of Chinese hamster ovary cells. Nucleic acid encoding sialidase is obtained using an oligonucleotide probe designed using amino acid sequence data on the sialidase.
Claims19
A recombinant cell line wherein a constitutive sialidase gene is not functionally expressed.
Substantially homogeneous sialidase, obtainable from cell culture fluid of a Chinese hamster ovary cell line.
A nucleic acid sequence encoding a sialidase, obtained by a process comprising:
- (a) preparing an oligonucleotide probe encoding part or all of the amino acid sequence CRVQAQSPNSGLDFQDN;
- (b) hybridizing the probe with nucleic acid in a mammalian DNA library to form hybrids;
- (c) isolating hybrids, thereby obtaining a said nucleic acid sequence encoding a sialidase.
An oligonucleotide probe useful in obtaining a sialidase-encoding gene and having a sequence encoding part or all of the amino acid sequence CRVQAQSPNSGLDFQDN.
A process for the production of a glycoprotein, comprising expressing nucleic acid encoding the glycoprotein in a host cell wherein a constitutive sialidase gene is not functionally expressed.
Dependencies are inferred from the claim text.