Epoxide hydrolases, nucleic acids encoding them and methods for making and using them

An isolated or recombinant nucleic acid, wherein the nucleic acid comprises a sequence that hybridizes under stringent conditions to a nucleic acid comprising

• a sequence as set forth in SEQ ID NO:1,
• a sequence as set forth in SEQ ID NO:3,
• a sequence as set forth in SEQ ID NO:5,
• a sequence as set forth in SEQ ID NO:7,
• a sequence as set forth in SEQ ID NO:9,
• a sequence as set forth in SEQ ID NO:11,
• a sequence as set forth in SEQ ID NO:13,
• a sequence as set forth in SEQ ID NO:15,
• a sequence as set forth in SEQ ID NO:17,
• a sequence as set forth in SEQ ID NO:19,
• a sequence as set forth in SEQ ID NO:21,
• a sequence as set forth in SEQ ID NO:23,
• a sequence as set forth in SEQ ID NO:25,
• a sequence as set forth in SEQ ID NO:27,
• a sequence as set forth in SEQ ID NO:29,
• a sequence as set forth in SEQ ID NO:31,
• a sequence as set forth in SEQ ID NO:33,
• a sequence as set forth in SEQ ID NO:35,
• a sequence as set forth in SEQ ID NO:37,
• a sequence as set forth in SEQ ID NO:39,
• a sequence as set forth in SEQ ID NO:41,
• a sequence as set forth in SEQ ID NO:43,
• a sequence as set forth in SEQ ID NO:45,
• a sequence as set forth in SEQ ID NO:47,
• a sequence as set forth in SEQ ID NO:49,
• a sequence as set forth in SEQ ID NO:51,
• a sequence as set forth in SEQ ID NO:53,
• a sequence as set forth in SEQ ID NO:55,
• a sequence as set forth in SEQ ID NO:57,
• a sequence as set forth in SEQ ID NO:59,
• a sequence as set forth in SEQ ID NO:61,
• a sequence as set forth in SEQ ID NO:63,
• a sequence as set forth in SEQ ID NO:65,
• a sequence as set forth in SEQ ID NO:67,
• a sequence as set forth in SEQ ID NO:69,
• a sequence as set forth in SEQ ID NO:71,
• a sequence as set forth in SEQ ID NO:73,
• a sequence as set forth in SEQ ID NO:75,
• a sequence as set forth in SEQ ID NO:77, or
• a sequence as set forth in SEQ ID NO:79,
• wherein the nucleic acid encodes a polypeptide having an epoxide hydrolase activity.

A nucleic acid probe for identifying a nucleic acid encoding a polypeptide with an epoxide hydrolase activity, wherein the probe comprises at least 10 consecutive bases of a sequence comprising:

• a sequence as set forth in SEQ ID NO:1,
• a sequence as set forth in SEQ ID NO:3,
• a sequence as set forth in SEQ ID NO:5,
• a sequence as set forth in SEQ ID NO:7,
• a sequence as set forth in SEQ ID NO:9,
• a sequence as set forth in SEQ ID NO:11,
• a sequence as set forth in SEQ ID NO:13,
• a sequence as set forth in SEQ ID NO:15,
• a sequence as set forth in SEQ ID NO:17,
• a sequence as set forth in SEQ ID NO:19,
• a sequence as set forth in SEQ ID NO:21,
• a sequence as set forth in SEQ ID NO:23,
• a sequence as set forth in SEQ ID NO:25,
• a sequence as set forth in SEQ ID NO:27,
• a sequence as set forth in SEQ ID NO:29,
• a sequence as set forth in SEQ ID NO:31,
• a sequence as set forth in SEQ ID NO:33,
• a sequence as set forth in SEQ ID NO:35,
• a sequence as set forth in SEQ ID NO:37,
• a sequence as set forth in SEQ ID NO:39,
• a sequence as set forth in SEQ ID NO:41,
• a sequence as set forth in SEQ ID NO:43,
• a sequence as set forth in SEQ ID NO:45,
• a sequence as set forth in SEQ ID NO:47,
• a sequence as set forth in SEQ ID NO:49,
• a sequence as set forth in SEQ ID NO:51,
• a sequence as set forth in SEQ ID NO:53,
• a sequence as set forth in SEQ ID NO:55,
• a sequence as set forth in SEQ ID NO:57,
• a sequence as set forth in SEQ ID NO:59,
• a sequence as set forth in SEQ ID NO:61,
• a sequence as set forth in SEQ ID NO:63,
• a sequence as set forth in SEQ ID NO:65,
• a sequence as set forth in SEQ ID NO:67,
• a sequence as set forth in SEQ ID NO:69,
• a sequence as set forth in SEQ ID NO:71,
• a sequence as set forth in SEQ ID NO:73,
• a sequence as set forth in SEQ ID NO:75,
• a sequence as set forth in SEQ ID NO:77,
• a sequence as set forth in SEQ ID NO:79
• wherein the probe identifies the nucleic acid by binding or hybridization.

A nucleic acid probe for identifying a nucleic acid encoding a polypeptide having an epoxide hydrolase activity, wherein the probe comprises a nucleic acid comprising

• a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by visual inspection.

An amplification primer sequence pair for amplifying a nucleic acid encoding a polypeptide having a epoxide hydrolase activity, wherein the primer pair is capable of amplifying a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:11, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

A method of amplifying a nucleic acid encoding a polypeptide having an epoxide hydrolase activity comprising amplification of a template nucleic acid with an amplification primer sequence pair capable of amplifying a nucleic acid sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

An expression cassette comprising a nucleic acid comprising:

• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

A vector comprising a nucleic acid comprising

• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:11, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

A transformed cell comprising a vector, wherein the vector comprises

• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:11, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

A transformed cell comprising

• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

A transgenic non-human animal comprising

• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:11, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

A transgenic plant comprising

• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:11, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

A transgenic seed comprising

• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

An antisense oligonucleotide comprising a nucleic acid sequence complementary to or capable of hybridizing under stringent conditions to

• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:11, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

A method of inhibiting the translation of an epoxide hydrolase message in a cell comprising administering to the cell or expressing in the cell an antisense oligonucleotide comprising a nucleic acid sequence complementary to or capable of hybridizing under stringent conditions to a nucleic acid comprising

• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

An isolated or recombinant polypeptide comprising

• (a) a polypeptide comprising
• an amino acid sequence having at least 50% identity to SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:46, SEQ ID NO:48, SEQ ID NO:54, SEQ ID NO:58, SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:64, SEQ ID NO:68, SEQ ID NO:70, SEQ ID NO:72, SEQ ID NO:74, SEQ ID NO:76, SEQ ID NO:78, or SEQ ID NO:80 over a region of at least about 100 residues,
• an amino acid sequence having at least 60% identity to SEQ ID NO:10, SEQ ID NO:24, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:40, SEQ ID NO:50, SEQ ID NO:52, SEQ ID NO:56, or SEQ ID NO:66 over a region of at least about 100 residues,
• an amino acid sequence having at least 70% identity to SEQ ID NO:26, or SEQ ID NO:38 over a region of at least about 100 residues, or
• (b) a polypeptide encoded by a nucleic acid comprising
• (i) a nucleic acid sequence having at least 50% sequence identity to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, or SEQ ID NO:79 over a region of at least about 100 residues,
• a nucleic acid sequence having at least 60% sequence identity to SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:39, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:55, or SEQ ID NO:65 over a region of at least about 100 residues, or
• a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:25, or SEQ ID NO:37 over a region of at least about 100 residues,
• wherein the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;
• or,
• (ii) a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:3, or a subsequence thereof, a sequence as set forth in SEQ ID NO:5, or a subsequence thereof, a sequence as set forth in SEQ ID NO:7, or a subsequence thereof, a sequence as set forth in SEQ ID NO:9, or a subsequence thereof, a sequence as set forth in SEQ ID NO:1, or a subsequence thereof, a sequence as set forth in SEQ ID NO:13, or a subsequence thereof, a sequence as set forth in SEQ ID NO:15, or a subsequence thereof, a sequence as set forth in SEQ ID NO:17, or a subsequence thereof, a sequence as set forth in SEQ ID NO:19, or a subsequence thereof, a sequence as set forth in SEQ ID NO:21, or a subsequence thereof, a sequence as set forth in SEQ ID NO:23, or a subsequence thereof, a sequence as set forth in SEQ ID NO:25, or a subsequence thereof, a sequence as set forth in SEQ ID NO:27, or a subsequence thereof, a sequence as set forth in SEQ ID NO:29, or a subsequence thereof, a sequence as set forth in SEQ ID NO:31, or a subsequence thereof, a sequence as set forth in SEQ ID NO:33, or a subsequence thereof, a sequence as set forth in SEQ ID NO:35, or a subsequence thereof, a sequence as set forth in SEQ ID NO:37, or a subsequence thereof, a sequence as set forth in SEQ ID NO:39, or a subsequence thereof, a sequence as set forth in SEQ ID NO:41, or a subsequence thereof, a sequence as set forth in SEQ ID NO:43, or a subsequence thereof, a sequence as set forth in SEQ ID NO:45, or a subsequence thereof, a sequence as set forth in SEQ ID NO:47, or a subsequence thereof, a sequence as set forth in SEQ ID NO:51, or a subsequence thereof, a sequence as set forth in SEQ ID NO:53, or a subsequence thereof, a sequence as set forth in SEQ ID NO:55, or a subsequence thereof, a sequence as set forth in SEQ ID NO:57, or a subsequence thereof, a sequence as set forth in SEQ ID NO:59, or a subsequence thereof, a sequence as set forth in SEQ ID NO:61, or a subsequence thereof, a sequence as set forth in SEQ ID NO:63, or a subsequence thereof, a sequence as set forth in SEQ ID NO:65, or a subsequence thereof, a sequence as set forth in SEQ ID NO:67, or a subsequence thereof, a sequence as set forth in SEQ ID NO:69, or a subsequence thereof, SEQ ID NO:71, or a subsequence thereof, SEQ ID NO:73, or a subsequence thereof, SEQ ID NO:75, or a subsequence thereof, SEQ ID NO:77, or a subsequence thereof, SEQ ID NO:79, or a subsequence thereof.

A method for isolating or recovering a nucleic acid encoding a polypeptide with an epoxide hydrolase activity from an environmental sample comprising the steps of:

• (a) providing an amplification primer sequence pair for amplifying a nucleic acid encoding a polypeptide with an epoxide hydrolase activity, wherein the primer pair is capable of amplifying SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53, SEQ ID NO:55, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:65, SEQ ID NO:67, SEQ ID NO:69, or a subsequence thereof;
• (b) isolating a nucleic acid from the environmental sample or treating the environmental sample such that nucleic acid in the sample is accessible for hybridization to the amplification primer pair;
• and,
• (c) combining the nucleic acid of step (b) with the amplification primer pair of step (a) and amplifying nucleic acid from the environmental sample, thereby isolating or recovering a nucleic acid encoding a polypeptide with an epoxide hydrolase activity from an environmental sample.

A method for producing a library of nucleic acids encoding a plurality of modified epoxide hydrolase active sites or substrate binding sites, wherein the modified active sites or substrate binding sites are derived from a first nucleic acid comprising a sequence encoding a first active site or a first substrate binding site the method comprising the following steps:

• (a) providing a first nucleic acid encoding a first active site or first substrate binding site, wherein the first nucleic acid sequence comprises a sequence that hybridizes under stringent conditions to a sequence as set forth in SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53, SEQ ID NO:55, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:65, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, SEQ ID NO:79, or a subsequence thereof, and the nucleic acid encodes a epoxide hydrolase active site or an epoxide hydrolase substrate binding site;
• (b) providing a set of mutagenic oligonucleotides that encode naturally-occurring amino acid variants at a plurality of targeted codons in the first nucleic acid;
• and,
• (c) using the set of mutagenic oligonucleotides to generate a set of active site-encoding or substrate binding site-encoding variant nucleic acids encoding a range of amino acid variations at each amino acid codon that was mutagenized, thereby producing a library of nucleic acids encoding a plurality of modified epoxide hydrolase active sites or substrate binding sites.

An isolated nucleic acid molecule selected from the group consisting of:

• (a) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:81;
• (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:82;
• (c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:83;
• (d) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:84;
• (e) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:85;
• (f) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:86;
• (g) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:87;
• (h) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:88;
• (i) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:89;
• (j) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:90;
• (k) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:91;
• and
• (e) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:92.

An isolated polypeptide selected from the group consisting of:

• a) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 or 92, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 or 92;
• b) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 or 92;
• and
• c) a polypeptide comprising an amino acid sequence which is at least 60% homologous to the amino acid sequence of SEQ ID NO:81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 or 92.