Method for producing L-amino acid using bacteria belonging to the genus escherichia
AssigneeAjinomoto Co., Inc.
InventorsVitaliy Arkadyevich Livshits, Maria Viacheslavovna Vitushkina, Mikhail Markovich Gusyatiner, Mikhail Kharisovich Ziyatdinov, Valery Zavenovich Akhverdian, Ekaterina Alekseevna Savrasova, Vera Georgievna Doroshenko, Sergey Vladimirovich Mashko
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Abstract
A method for producing an L-amino acid, such as L-phenylalanine, L-threonine is provided using bacterium belonging to the genus Escherichia wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of protein encoded by the yedA gene.
Claims10
An L-amino acid producing bacterium belonging to the genus Escherichia wherein the L-amino acid production by said bacterium is enhanced by enhancing activity of a protein as defined in the following (A) or (B) in a cell of said bacterium:
- (A) a protein which comprises the amino acid sequence shown in SEQ ID NO:2 in Sequence listing;
- (B) a protein which comprises an amino acid sequence including deletion, substitution, insertion or addition of one or several amino acids in the amino acid sequence shown in SEQ ID NO:2 in Sequence listing, and which has an activity of making bacterium having enhanced resistance to an L-amino acid and/or an amino acid analog.
The method according to claim 10, further comprising
- esterifying L-phenylalanine to generate a lower alkyl ester of L-phenylalanine,
- condensing the lower alkyl ester of L-phenylalanine with the aspartic acid derivative, wherein the derivative is N-acyl-L-aspartic anhydride,
- separating the lower alkyl ester of N-acyl-α-L-aspartyl-L-phenylalanine from the reaction mixture, and
- hydrogenating the lower alkyl ester of N-acyl-α-L-aspartyl-L-phenylalanine to generate the lower alkyl ester of α-L-aspartyl-L-phenylalanine.
Dependencies are inferred from the claim text.