Mutant aprE promoter
AssigneeGenencor International, Inc.
InventorsFernando Valle, Eugenio Ferrari
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Abstract
The present invention provides a mutant aprE promoter and methods for the production of a desired protein in a Bacillus host cell, which comprises the mutant aprE promoter. The present invention provides the sequence of a preferred aprE mutant promoter, which provided for a 100-fold increase in the production of a protein from Bacillus subtilis.
Claims20
An isolated mutant aprE promoter.
The isolated mutant aprE promoter having the nucleotide sequence as given in SEQ ID NO:
- 1.
An expression vector comprising an isolated mutant aprE promoter.
A host cell comprising an isolated mutant aprE promoter.
A method for producing a desired protein in a Bacillus comprising, culturing a Bacillus comprising nucleic acid encoding the desired protein said Bacillus further comprising an isolated mutant aprE promoter and optionally recovering the protein.
Dependencies are inferred from the claim text.