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AssigneeRohm Enzyme Finland Oy
InventorsArja Mantyla, Marja Paloheimo, Raija Lantto, Richard Fagerstrom, Tarja Lahtinen, Pirkko Suominen, Jari Vehmaanpera
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The present invention is related to an improved production of bacterial proteins in filamentous fungus, e.g. in Tfichodenna and Aspergillus. The improvement is achieved by constructing expression vectors, which comprise the bacterial protein encoding DNA sequences fused in frame with a DNA sequence encoding a filamentous fungus secretable protein or one or more functional domains of said protein. Filamentous fungus hosts transformed with such expression vectors secrete the desired proteins or enzymes, especially xylanases or cellulases originating from bacteria or more preferably from actinornycetes into the culture medium of the host. The desired proteins or enzymes can be used directly from the culture medium after separation of host cells or recovered and treated using down-stream processes, which are appropriate for the respective application. Xylanases or cellulases from actinomycetes produced using the above expression vectors are most suitable for treating plant derived materials e.g. in pulp and paper industries.
A recombinant expression vector for production of bacterial proteins in a filamentous fungal host comprising a promoter operably linked to a DNA sequence of a filamentous fungus secretable protein or one or more functional domains of said protein, which is fused in frame with a DNA sequence encoding a bacterial protein.
A method for producing a preparation containing an enzyme originating from bacteria preferably from actinomycete in a filamentous fungal host comprising the steps of:
An isolated DNA sequence encoding the amino acid sequence of Actinomadura xylanase, comprising the amino acid sequence of SEQ ID NO:
An isolated DNA fragment of a plasmid comprising the DNA sequences selected from the group consisting of pALK923 (DSM9322), pALK938 (DSM9899), pALK939 (DSM9900), pALK940 (DSM9901), pALK941 (DSM9902) and pALK1056 (DSM9903) that encodes Actinomadura flexuosa AM35 xylanase and equivalents thereof.
An isolated DNA fragment of plasmid comprising the DNA sequences selected from the plasmids selected from a group consisting of plasmid pALK927 (DSM9447) or plasmid pALK928 (DSM9448) that encodes the Actinomadura flexuosa AM50 xylanase and equivalents thereof.
Dependencies are inferred from the claim text.