Promoters for expressing genes in a fungal cell

An isolated nucleic acid sequence, selected from the group consisting of:

• (a) a nucleic acid sequence encoding a polypeptide having an amino acid sequence which has at least 65% identity with amino acids 22 to 581 of SEQ ID NO. 4, amino acids 19 to 200 of SEQ ID NO. 5, or amino acids 1 to 187 of SEQ ID NO. 6;
• (b) a nucleic acid sequence having at least 65% homology with nucleotides 4013 to 5743 of SEQ ID NO. 1, nucleotides 993 to 1593 of SEQ ID NO. 2, or nucleotides 3061 to 3678 of SEQID NO. 3;
• (c) a nucleic acid sequence which hybridizes under very low, low, medium, medium-high, high, or very high stringency conditions with (i) nucleotides 4013 to 5743 of SEQ ID NO. 1, nucleotides 993 to 1593 of SEQ ID NO. 2, or nucleotides 3061 to 3678 of SEQ ID NO. 3;
• (ii) the cDNA sequence contained in nucleotides 4013 to 5743 of SEQ ID NO. 1, nucleotides 993 to 1593 of SEQ ID NO. 2, or nucleotides 3061 to 3678 of SEQ ID NO. 3;
• (iii) a subsequence of (i) or (ii) of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii), or (iii);
• (d) a nucleic acid sequence encoding a variant of the polypeptide having an amino acid sequence of SEQ ID NO. 4, SEQ ID. NO. 5, or SEQ ID NO. 6 comprising a substitution, deletion, and/or insertion of one or more amino acids;
• (e) an allelic variant of (a), (b), or (c);
• and
• (f) a subsequence of (a), (b), (c), or (e).

A method for producing a polypeptide, comprising:

• (a) cultivating a fungal host cell in a medium for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter foreign to the first nucleic acid sequence, wherein the promoter comprises a nucleic acid sequence selected from the group consisting of (i) nucleotides 1 to 3060 of SEQ ID NO:3;
• (ii) a subsequence of (i) that retains the promoter activity of nucleotides 1 to 3060 of SEQ ID NO:3;
• and (iii) a nucleic acid sequence that hybridizes under medium stringency conditions with nucleotides 1 to 3060 of SEQ ID NO:3;
• and
• (b) isolating the polypeptide from the cultivation medium.