Process for producing hydroxynitrile lyases
AssigneeJulich Chiral Solutions GmbH
InventorsFranz Effenberger, Harald Wajant, Siegfried Forster
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Abstract
The present invention relates to an improved process for producing hydroxynitrile lyases.
Claims11
Process for producing a hydroxynitrile lyase in dissolved form, where bacteria of the Escherichia coli species, containing at least one gene, coding a hydroxynitrile lyase, are precultivated in a culture medium at 30 to 40 degrees Centigrade, induced with 1 to 100 uM IPTG (end concentration in the culture medium), and cultivated and released at 15 to 25 degrees Centigrade, and then dissolved hydroxynitrile lyase is isolated.
Process, as claimed in any one of the preceding claims, wherein the cultivation takes place in an LB medium.
Process, as claimed in any one of the preceding claims, wherein after the cultivation and prior to release, the bacteria are separated from the culture medium.
Process, as claimed in any one of the preceding claims, wherein cell lysis takes place by means of ultrasound.
Process, as claimed in any one of the preceding claims, wherein the gene, coding the hydroxynitrile lyase, is present in the bacteria on a plasmid.
Process, as claimed in any one of the preceding claims, wherein the hydroxynitrile lyase is a naturally occurring hydroxynitrile lyase or a mutant variant thereof.
Process, as claimed in any one of the preceding claims, wherein the hydroxynitrile lyase is derived from Sorghum bicolor, Manihot esculenta, Hevea brasiliensis, Linum usitatissimum or Prunus sp.
Dependencies are inferred from the claim text.