Proteolytic fermenter
AssigneeGreen Earth Industries
InventorsAlexander V. Pyntikov, Salvatore P. Salerno
Life of this patent
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Abstract
A process of controlling the production of enzymatic protein hydrolysate from cold water animals, containing up to 90% free amino acids, cold fish oil, hydroxilaptite (calcium orthophosphate), proteins, peptides, vitamin, minerals and calcium salts of phosphorus. The process provides a greater degree of refining than existing technology through the use of acid and alkaline denaturation steps, in combination with advanced separation and drying technology. The new drying technology results in a higher-quality, less expensive mixture than was previously possible.
Claims20
A method for producing a profound enzymatic protein hydrolysate, comprising:
- preparing ferments and substrate made from cold water marine animals;
- feeding said ferments and said substrate, water, and an alkaline solution to a piscean bioreactor;
- heating and stirring the contents of said bioreactor to form a fish hydrolysate;
- purifying said hydrolysate;
- and
- concentrating said hyrolysate so as to produce an EPH comprising 70 to 90% free amino acids, 10 to 20% highly molecular peptides, and a remainder of nutrients.
A method for producing an enzymatic protein hydrolysate, comprising:
- feeding raw materials into a feed processor having a multi-ton capacity;
- cutting up and mixing said raw materials in said feed processor to form a slurry;
- feeding said slurry into an alkaline-based reactor to form a hydrolysate;
- centrifuging said hydrolysate to produce an hydroxylapatite and a fish protein mix;
- acid denaturating the fish protein mix to form an acid denaturated fish protein mix;
- centrifuging the denaturated fish protein mix to produce cold fish oil and fish protein;
- alkaline denaturating and centrifuging said fish protein to produce a peptide mix and phosphoric salts of calcium;
- preserving the peptide mix with a mild acid;
- and
- concentrating the final product to form an EPH that primarily includes free amino acids.
A computer method for establishing the quantity of water to be used in a piscean bioreactor, comprising:
- inputing into a central control system raw material identifiers, comprising:
- a substrate fish type and its mass;
- a ferment source and its mass;
- storing said raw material identifiers;
- and
- outputing onto a display panel the mass of water required to operate the piscean bioreactor.
A computer method for preparing raw materials to be used in a piscean bioreactor, comprising:
- inputing into a central control system raw material identifiers, comprising:
- a substrate fish type and its mass;
- a ferment source and its mass;
- storing said raw material identifiers;
- and
- outputting the mass content of water required to operate the bioreactor.
A computerized method for operation of a piscean bioreactor, comprising:
- inputing raw material identifiers, comprising:
- selecting the type of fish used;
- inputing the total weight of raw materials to be used;
- storing raw material identifiers;
- receiving the appropriate quantity of water;
- receiving pH for the bioreaction mixture;
- receiving the temperature of the bioreaction mixture;
- receiving the bioreaction time;
- receiving the concentration of amino nitrogen in the bioreaction mixture;
- receiving the pressure of the bioreaction vessel;
- adjusting the pH of the bioreaction mixture;
- adjusting the temperature of the bioreaction mixture;
- adjusting pressure relief valves of the bioreactor;
- and
- adjusting status indication for the bioreactor.
Dependencies are inferred from the claim text.