Comparative phenotype analysis

A method for comparing the function of a gene in at least two cell preparations, comprising the steps of:

• a) providing a testing device comprising a plurality of testing wells, wherein said wells contain one or more gel-initiating agents, and at least one testing substrate selected from the group consisting of nitrogen sources, phosphorus sources, sulfur sources, and auxotrophic supplements;
• b) preparing a first suspension comprising a first cell preparation, in an aqueous solution comprising a gelling agent, and a second suspension comprising a second cell preparation, in an aqueous solution comprising a gelling agent, under conditions such that said first and second suspensions remain ungelled;
• c) introducing said first and second suspension into said wells of said testing device under conditions such that said first and second suspensions form a gel matrix within said wells, such that said first and second cell preparations are within said gel matrix;
• d) detecting the response of said first and second cell preparations to said testing substrate;
• and
• e) comparing the response of said first and second cell preparations.

A kit for determining the phenotype of at least two organisms, comprising:

• i) a testing device containing a plurality of wells, wherein said wells contain one or more testing substrates selected from the group consisting of nitrogen sources, phosphorus sources, sulfur sources, and auxotrophic supplements;
• ii) a first aqueous suspension;
• and
• iii) a second aqueous suspension.