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OwnerDavid John King, Andrew Mountain +2inventors
InventorsDAVID JOHN KING, ANDREW MOUNTAIN, RAYMOND JOHN OWENS, GEOFFREY THOMAS YARRANTON
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A multivalent antigen-binding protein comprises a first Fv fragment bound to at least one further Fv fragment by a connecting structure which links the Fv fragments to each other but which maintains them spaced apart such that the proteins are capable of binding to adjacent antigenic determinants. Typically the connecting structure comprises a spacing polypeptide sequence, which may be about 3 to 16 amino acids in length, connected to a linkage unit which may be a synthetic chemical linker, e.g., a maleimide linker, or is a polypeptide sequence leading from the spacing sequence. In a particularly preferred embodiment, the multivalent antigen binding protein comprises a VH domain having attached to its C-terminal end a V-C joining sequence and an antibody hinge sequence. Preferably one or more of the Fv fragments is a single chain Fv (scFv). The proteins are preferably prepared by recombinant DNA techniques and are useful for in vivo therapeutic and especially diagnostic applications.
A multivalent antigen binding protein comprising a first Fv fragment bound to at least one further Fv fragment by a connecting structure which links the Fv fragments to each other but which maintains them spaced apart such that the protein is capable of binding to adjacent antigenic determinants.
A multivalent antigen-binding protein comprising a VH domain having attached to its C-terminal end a joining sequence and an antibody hinge sequence, the VH domain associated in a heterodimer with a VL domain, and the VS-VL heterodimer being linked to a similar VH-VL heterodimer by disulphide bonds formed between the hinge recion cysteine residues.
A protein according to any one of the preceding claims, comprising a connecting structure selected from the following amino acid sequences (i), (ii) or (iii).
A protein according to any one of the preceding claims which has only one specificity.
DNA coding for a VL or preferably VH domain polypeptide having a spacing polypeptide sequence and preferably a linkage unit polypeptide sequence attached to one end, preferably the C-terminal end, thereof.
An antibody variable domain, preferably a VH domain, having fused onto one of its ends, preferably its C-terminal end, a spacing polypeptide sequence.
Dependencies are inferred from the claim text.