Patched genes and uses related thereto
AssigneeStanford University
InventorsMatthew P. Scott, Lisa V. Goodrich, Ronald L. Johnson
Life of this patent
Estimating the term…
Abstract
Invertebrate and vertebrate patched genes are provided, including the mouse and human patched genes, as well as methods for isolation of related genes, where the genes may be of different species or in the same family. Having the ability to regulate the expression of the patched gene, allows for the elucidation of embryonic development, cellular regulation associated with signal transduction by the patched gene, the identification of agonist and antagonist to signal transduction, identification of ligands for binding to patched, isolation of the ligands, and assaying for levels of transcription and expression of the patched gene.
Claims22
A DNA sequence other than present in a chromosome encoding a patched gene other than the Drosophila patched gene or fragment thereof of at least about 12 bp different from the sequence of the Drosophila patched gene.
A cell comprising an expression cassette comprising a transcriptional initiation region functional in an expression host, said transcriptional initiation region consisting of a 5′ non-coding region regulating the transcription of patched protein comprising the promoter and enhancer, a marker gene, and a transcriptional termination region, as part of an extrachromosomal element or integrated into the genome of a host cell as a result of introduction of said expression cassette into said host, and the cellular progeny thereof.
A method for following embryonic development employing the patched protein in an embryo, said method comprising:
- integrating an expression cassette comprising a transcriptional initiation region functional in embryonic host cells, said transcriptional initiation region consisting of a 5′ non-coding region regulating the transcription of patched protein, a marker gene, and a transcriptional termination region, wherein said embryonic host cells are capable of developing into a fetus;
- growing said embryonic host cells, whereby proliferation and differentiation occur;
- and
- locating cells comprising expression of the patched protein by means of expression of said marker gene.
A method for screening candidate compounds for agonist activity with the patched protein, said method comprising:
- combining said candidate protein with a vertebrate or invertebrate cell comprising said patched protein in the membrane of said cell and an expression cassette comprising a transcriptional initiation region functional in an expression host, said transcriptional initiation region consisting of a 5′ non-coding region regulating the transcription of patched protein, a marker gene, and a transcriptional termination region, as part of an extrachromosomal element or integrated into the genome of a host cell;
- and
- assaying for the expression of said marker gene.
A monoclonal antibody binding specifically to a patched protein, other than the Drosophila patched protein.
Dependencies are inferred from the claim text.