More protein-protein interactions in the inner ear
AssigneeHybrigenics
InventorsLaurent Daviet, Pierre Legrain, Christine Petit, Batiste Boeda, Aziz El-Amraoui
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Abstract
The present invention relates to protein-protein interactions involved in deafness or in hearing disorders and/or diseases. More specifically, the present invention relates to complexes of polypeptides or polynucleotides encoding the polypeptides, fragments of the polypeptides, antibodies to the complexes, Selected Interacting Domains (SID®) which are identified due to the protein-protein interactions, methods for screening drugs for agents which modulate the interaction of proteins and pharmaceutical compositions that are capable of modulating the protein-protein interactions.
Claims15
A complex of protein-protein interactions as defined in columns 1 and 3 of Table 2.
A complex of polynucleotides as defined in SEQ ID Nos. 1 or 2 encoding for the polypeptides.
A recombinant host cell expressing the interacting polypeptides as defined in Table 1.
A method for selecting a modulating compound comprising:
- (a) cultivating a recombinant host cell with a modulating compound on a selective medium and a reporter gene the expression of which is toxic for said recombinant host cell wherein said recombinant host cell is transformed with two vectors:
- (i) wherein said first vector comprises a polynucleotide in column 1 of Table 2 encoding a first hybrid polypeptide and a DNA bonding domain;
- (ii) wherein said second vector comprises a polynucleotide in column 3 of Table 2 encoding a second hybrid polypeptide and an activating domain that activates said toxic reporter gene when the first and second hybrid polypeptides interact;
- (b) selecting said modulating compound which inhibits the growth of said recombinant host cell.
A vector comprising the polynucleotide comprising the SEQ ID Nos. 1 or 2.
A fragment of a polypeptide comprising SEQ ID Nos. 3 or 4.
A variant of a polypeptide comprising SEQ ID Nos. 3 or 4.
A polynucleotide comprising SEQ ID Nos. 5 and 6 or a fragment or variant thereof.
A polypeptide of SEQ ID Nos. 7 and 8 or a fragment or variant thereof.
A method to detect Usher type I syndrome said method comprising:
- obtaining a biological sample from a subject;
- and
- identifying a defect in the proteins which are myosin VIIa, harmonin b and cadherin 23.
Dependencies are inferred from the claim text.