Diagnostic microarray and method of use thereof

A method of detecting one or more target molecules in a sample solution, the method comprising:

• (a) electrophoretically transporting the sample solution through a liquid permeable layer, wherein the liquid permeable layer includes at least one microregion having a plurality of microbeads embedded in the liquid permeable layer;
• the microbeads having a plurality of target probes on their surfaces;
• wherein the target probes are capable of specifically binding to designated target molecules to form target probe/target molecule complexes;
• and
• (b) detecting the target probe/target molecule complexes.

A method of detecting a target molecule in a sample comprising:

• (a) introducing a first low conductivity buffer solution including the sample into a liquid chamber;
• (b) electrophoretically transporting the first low conductivity buffer solution through a liquid permeable layer which is in fluid connection with the liquid chamber;
• wherein the liquid permeable layer includes at least one microregion having a plurality of microbeads embedded in the liquid permeable layer;
• the microbeads having a plurality of a target probe on their surfaces;
• the target probe being capable of specifically binding to the target molecule to form a target molecule/target probe complex;
• (c) introducing a second low conductivity buffer solution into the liquid chamber;
• wherein the second low conductivity buffer solution includes a fluorescently labeled target molecule;
• (d) electrophoretically transporting the second low conductivity buffer solution through the liquid permeable layer to form a fluorescent target molecule/target probe complex;
• and
• (e) detecting the fluorescent target molecule/target probe complex.

A kit for the analysis of biological samples comprising:

• (a) a microarray device comprising a liquid permeable layer including a plurality of microregions, each microregion including a plurality of microbeads embedded in the liquid permeable layer;
• wherein the microbeads have a plurality of target probes on their surfaces and the microbeads in a given microregion have a plurality of the target probes on their surfaces;
• (b) a low conductivity buffer solution;
• and
• (c) a buffer solution including a set of visualization probes.

A method of detecting a target molecule in a sample comprising:

• (a) introducing a plurality of a visualization probe into a low conductivity solution including the sample to form a labeled solution;
• wherein the visualization probe is capable of specifically binding to the target molecule to form a labeled target molecule;
• (b) electrophoretically transporting the labeled solution through a liquid permeable layer;
• wherein the liquid permeable layer includes at least one microregion having a plurality of microbeads embedded in the liquid permeable layer;
• the microbeads having a plurality of a target probe on their surfaces;
• wherein the target probe is capable of specifically binding to the labeled target molecule to form a labeled target molecule/target probe complex on a microbead surface;
• and
• (c) detecting the bound labeled target molecule/target probe complex.