Detecting targets by unique identifier nucleotide tags
OwnerLi Shen, Xiang Yu +1inventors
InventorsLi Shen, Xiang Yu, Hui Cen
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Abstract
This invention relates generally to the field of target detection. In particular, the present inventions provides for methods and compositions for assaying a plurality of different non-nucleic acid targets or for assaying activities of a plurality of enzymes using, inter alia, oligonucleotide identification (ID) tags.
Claims62
A method for assaying a plurality of different non-nucleic acid targets in a sample, which method comprises:
- a) providing a plurality of reporter ligands, each said reporter ligand comprising a portion that specifically binds to a target present or suspected being present in a sample and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said reporter ligands are distinguishable from each other based on an identifiable property other than the length of said oligonucleotide ID tags;
- b) contacting said sample with said plurality of reporter ligands provided in step a) under suitable conditions to allow binding between said targets, if present in said sample, to said plurality of reporter ligands;
- c) separating reporter ligands bound to said targets from unbound reporter ligands;
- and
- d) assessing the identity and/or quantity of targets in said sample by detecting and/or quantifying said oligonucleotide ID tags in said reporter ligands bound to said targets.
A composition for assaying a plurality of non-nucleic acid targets in a sample, which composition comprises a plurality of reporter ligands, each said reporter ligand comprising a portion that specifically binds to a target present or suspected being present in a sample and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said reporter ligands are distinguishable from each other based on an identifiable property other than the length of said oligonucleotide ID tags.
A composition, which composition comprises a plurality of complexes formed between a plurality of different non-nucleic acid targets and a plurality of corresponding reporter ligands, each said reporter ligand comprising a portion that specifically binds to a target and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said reporter ligands are distinguishable from each other based on an identifiable property other than the length of said oligonucleotide ID tags.
A method for assaying a plurality of different non-nucleic acid targets in a sample, which method comprises:
- a) providing a plurality of target antagonists, each said target antagonist comprising a portion that specifically binds to a corresponding receptor ligand and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said target antagonists are distinguishable from each other based on an identifiable property other than the length of said oligonucleotide ID tags;
- b) providing a plurality of receptor ligands, each receptor ligand specifically binding to a different target and its corespending reporter antagonist in a competitive manner;
- c) contacting a sample with said plurality of target antagonists and said plurality of receptor ligands provided in steps a) and b) under suitable conditions to allow competitive binding between said targets, if present in said sample, and their corespending reporter antagonists, to their corresponding receptor ligands;
- d) separating said target antagonists bound to said receptor ligands from said unbound target antagonists;
- and
- e) assessing the identity and/or quantity of targets in said sample by detecting and/or quantifying said oligonucleotide ID tags in target antagonists bound to said receptor ligands.
A composition for assaying a plurality of non-nucleic acid targets in a sample, which composition comprises a plurality of target antagonists, each said target antagonist comprising a portion that specifically binds to a corresponding receptor ligand and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said target antagonists are distinguishable from each other based on an identifiable property other than the length of said oligonucleotide ID tags.
A composition, which composition comprises a plurality of complexes formed between a plurality of receptor ligands and their corresponding target antagonist, wherein each said receptor ligand specifically binds to a different target or its corespending reporter antagonist in a competitive maimer and each said target antagonist comprising a portion that specifically binds to a corresponding receptor ligand and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said target antagonists are distinguishable from each other based on an identifiable property other than the length of said oligonucleotide ID tags.
A method for assaying a plurality of different non-nucleic acid targets in a cell, which method comprises:
- a) providing a plurality of target antagonists, each said target antagonist comprising a portion that specifically associates with a corresponding cellular component and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said target antagonists are distinguishable from each other based on an identifiable property other than the length of said oligonucleotide ID tags;
- b) delivering said plurality of target antagonists into said cell to allow competitive interaction between said targets, if present in said cell, and said target antagonists, with said cellular components;
- c) obtaining an equal amount of said cellular components associated with said targets or said target antagonists;
- and
- d) assessing the identity and/or quantity of targets in said cell by detecting and/or quantifying said oligonucleotide ID tags in said target antagonists associated with said cellular components.
A method for assaying activities of a plurality of enzymes in a sample, which method comprises:
- a) providing a plurality of reporter substrates, each said reporter substrate comprising a portion that can be modified by a corresponding enzyme in a sample and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said reporter substrates are distinguishable from each other;
- b) contacting said plurality of reporter substrates with said sample under suitable conditions to allow each enzyme to catalyze a modification reaction on its corresponding reporter substrate;
- c) separating modified reporter substrates from unmodified reporter substrates;
- and
- d) assessing the activities of said enzymes in said sample by detecting and/or quantifying said oligonucleotide ID tags in said modified reporter substrates.
A composition for assaying activities of a plurality of enzymes in a sample, which composition comprises a plurality of reporter substrates, each said reporter substrate comprising a portion that can be modified by a corresponding enzyme in a sample and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said reporter substrates are distinguishable from each other.
A composition, which composition comprises a plurality of reporter substrates, each said reporter substrate comprising a portion that has been modified by a corresponding enzyme in a sample and an oligonucleotide identification (ID) tag, wherein said oligonucleotide ID tags in said reporter substrates are distinguishable from each other.
Dependencies are inferred from the claim text.