AC methods for the detection of nucleic acids
AssigneeClinical Micro Sensors, Inc.
InventorsJon Faiz Kayyem, Stephen D. O'Connor
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Abstract
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.
Claims26
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising
- a) applying a first input signal comprising an AC component and a non-zero DC component to a hybridization complex, said hybridization complex comprising at least a target sequence and a first probe single stranded nucleic acid, said hybridization complex being covalently attached to:
- i) a first electron transfer moiety comprising an electrode;
- and
- ii) a second electron transfer moiety;
- and
- b) detecting the presence of said hybridization complex by receiving an output signal characteristic of electron transfer through said hybridization complex.
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising
- a) applying a first input signal comprising an AC component at a first frequency and a non-zero DC component to a hybridization complex, said hybridization complex comprising at least a target sequence and a first probe single stranded nucleic acid, said hybridization complex being covalently attached to:
- i) a first electron transfer moiety comprising an electrode;
- and
- ii) a second electron transfer moiety;
- b) applying a second input signal comprising an AC component at least a second frequency and a non-zero DC component to said hybridization complex;
- and
- c) detecting the presence of said hybridization complex by receiving an output signal characteristic of electron transfer through said hybridization complex.
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising
- a) applying a first input signal comprising an AC component and a first non-zero DC component to a hybridization complex, said hybridization complex comprising at least a target sequence and a first probe single stranded nucleic acid, said hybridization complex being covalently attached to:
- i) a first electron transfer moiety comprising an electrode;
- and
- ii) a second electron transfer moiety;
- b) applying a second input signal comprising said AC component and at least a second non-zero DC component to said hybridization complex;
- and
- c) detecting the presence of said hybridization complex by receiving an output signal characteristic of electron transfer through said hybridization complex.
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising
- a) applying a first input signal comprising an AC component at a first voltage amplitude
- to a hybridization complex, said hybridization complex comprising at least a target sequence and a first probe single stranded nucleic acid, said hybridization complex being covalently attached to:
- i) a first electron transfer moiety comprising an electrode;
- and
- ii) a second electron transfer moiety;
- b) applying a second input signal comprising said AC component at a second voltage amplitude to said hybridization complex;
- and
- c) detecting the presence of said hybridization complex by receiving an output signal characteristic of electron transfer through said hybridization complex.
An apparatus for the detection of target nucleic acids in a test sample, comprising:
- a) a test chamber comprising a first and a second measuring electrode, wherein said first measuring electrode comprises a covalently attached conductive oligomer covalently attached to a single stranded nucleic acid;
- and
- b) an AC/DC voltage source electrically connected to said test chamber.
An apparatus for the detection of target nucleic acids in a test sample, comprising:
- a) a test chamber comprising a first and a second measuring electrode, wherein said first measuring electrode comprises a covalently attached single stranded nucleic acid comprising a covalently attached second electron transfer moiety;
- and
- b) an AC/DC voltage source electrically connected to said test chamber.
Dependencies are inferred from the claim text.