Diagnosis of diseases associated with dna transcription
OwnerAlexander Olek, Christian Piepenbrock +1inventors
InventorsAlexander Olek, Christian Piepenbrock, Kurt Berlin
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Abstract
The present invention relates to the chemically modified genomic sequences of genes associated with DNA transcription to oligonucleotides and/or PNA-oligomers for detecting the cytosine methylation state of genes associated with DNA transcription which are directed against the sequence as well as to a method for ascertaining genetic and/or epigenetic parameters of genes associated with DNA transcription.
Claims32
A nucleic acid comprising a sequence at least 18 bases in length of a segment of the chemically pretreated DNA of genes associated with DNA transcription according to one of the sequences taken from the group of Seq. ID No.1 to Seq. ID No.346 and sequences complementary thereto.
A nucleic acid comprising a sequence at least 18 base pairs in length of a segment of the chemically pretreated DNA of genes associated with DNA transcription according to a sequence according to one of the genes ELF1 (M82882), ETV3 (L16464), ETV4 (D12765), TAF2C2 (Y09321), TCF9 (M29204), ZNF121 (M99593), ZNF131 (U09410), ZNF139 (U09848), ZNF154 (U20648), ZNF169 (U28322), ZNF2 (X60152), ZNF204 (AF033199), ZNF206 (AA206569), ZNF3 (X78926), ZNF37A (X69115), ZNF44 (X16281), ZNF8 (M29581), ZK1 (NM—005815), ADA (NM—000022), ATBF1 ((NM—006885), ATF3 (NM—001674), ZNF255 (NM—005774), CBFB(NM—001755), CEBPD (NM—005195), CTPS (NM—001905), DCTD (NM—001921), DR1 (NM—001938), ELF3 (NM—004433), ELK4 (NM—021795), ETV5 (NM—004454), KLF4 (NM—004235), FOXO1A (NM—002015), FLI1 (NM—002017), HHEX (NM—001529), HIVEP1 (NM—002114), HMG2 (NM—002129), ID1 (NM—002165), ID3 (NM—002167), LAF4 (NM—002285), ZNFN1A1 (NM—006060), LYL1 (NM—005583), MAFG (NM—002359), MAZ (NM—002383), ODC1 (NM—002539), PBX3 (NM—006195), POU2AF1 (NM—006235), POU2F2 (NM—002698), POU3F1 (NM—002699), PROP1 (NM—006261), RARG (NM—000966), RECQL (NM—002907), RXRA (NM—002957), SP100 (NM—003113), ZFP93 (NM—004234), MKRN3 (NM—005664), ZNF133 (NM—003434), ZNF157 (NM—003446), ZNF173 (NM—003449), ZNF189 (NM—003452), ZNF207 (NM—003457), ZNF262 (NM—005095), ZNF264 (NM—003417), ZNF74 (NM—003426), ZNF91 (NM—003430), POU3F2 (NM—005604), ZNF84 (NM—003428), ERV3, TCF8 and sequences complementary thereto.
A DNA- and/or PNA-array for analyzing diseases associated with the methylation state of genes, comprising at least one nucleic acid according to one of the preceeding claims.
Dependencies are inferred from the claim text.