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AssigneeJapan Tobacco Inc.
InventorsYoshimitsu Takakura, Tsuyoshi Inoue, Hideaki Saito, Toru Ito
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An object of the present invention is to provide a novel DNA sequence having a flower organ-specific promoter activity which makes it possible to express a foreign gene specifically in pistil or lodicule, this enabling genetic manipulation. The present invention provides a DNA fragment comprising the sequence of positions 1 to 5369 in the nucleotide sequence represented by SEQ ID NO:3 in Sequence Listing, the sequence of positions 3335 to 5108 therein, a part of these sequences or a sequence derived from these sequences by deletion, substitution, insertion or addition of one or more nucleotides and having a promoter activity; and a flower organ-specific promoter sequence which can be identified from among sequences obtained by screening a genomic library of rice or other plants by using as a probe the nucleotide sequence as described above or a part thereof.
A DNA fragment comprising the sequence of positions 3335 to 5108 in the nucleotide sequence represented by SEQ ID NO:3 in Sequence Listing, a part of said sequence or a sequence derived from said sequence by deletion, substitution, insertion or addition of one or more nucleotides and having a promoter activity.
A DNA fragment comprising the sequences of positions 3335 to 5108 in the nucleotide sequence represented by SEQ ID NO:3 in Sequence Listing or a sequence derived from said sequence by deletion, substitution, insertion or addition of one or more nucleotides and having a promoter activity.
A DNA fragment comprising the sequence of positions 1 to 5369 in the nucleotide sequence represented by SEQ ID NO:3 in Sequence Listing, a part of said sequence or a sequence derived from said sequence by deletion, substitution, insertion or addition of one or more nucleotides and having a promoter activity.
A DNA fragment comprising the sequence of positions 1 to 5369 in the nucleotide sequence represented by SEQ ID NO:3 in Sequence Listing or a sequence derived from said sequence by deletion, substitution, insertion or addition of one or more nucleotides and having a promoter activity.
A DNA fragment having a flower organ-specific promoter activity which is hybridizable with the sequence of positions 1 to 5369 in the nucleotide sequence represented by SEQ ID NO:3 in Sequence Listing or a part of said sequence having a flower organ-specific promoter activity.
A flower organ-specific promoter sequence which can be identified from among DNA sequences obtained by screening a genomic library of rice or another plant by using as a probe the nucleotide sequence represented by SEQ ID NO:1 in Sequence Listing or a part of said sequence.
A DNA fragment comprising a sequence having at least 15 consecutive nucleotides in the sequence from positions 22 to 1278 of the sequence represented by SEQ ID NO:1 in Sequence Listing or a nucleotide sequence complementary to said sequence.
A DNA fragment having a flower organ-specific promoter activity which is hybridizable with the sequence of positions 3335 to 5108 in the nucleotide sequence represented by SEQ ID NO:2 in Sequence Listing or a part of said sequence having a flower organ-specific promoter activity.
A DNA fragment having a flower organ-specific promoter activity which is hybridizable with the sequence of positions 3335 to 4994 in the nucleotide sequence represented by SEQ ID NO:2 in Sequence Listing or a part of said sequence having a flower organ-specific promoter activity.
A DNA fragment comprising a nucleic acid sequence of positions 3335 to 5108 in SEQ ID NO:3, wherein said DNA fragment has flower organ-specific promoter activity.
A DNA fragment comprising a nucleic acid sequence of positions 3335 to 4994 of SEQ ID NO:3, wherein said DNA fragment has flower organ-specific promoter activity.
Dependencies are inferred from the claim text.