Methods and compositions relating to plasmacytoid dendritic cells
OwnerGrayson B. Lipford, Hermann Wagner +1inventors
InventorsGrayson B. Lipford, Hermann Wagner, Martin Zenke
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Abstract
The invention provides methods and compositions relating to a dendritic cell expression database.
Claims82
A method for identifying a plasmacytoid dendritic cell comprising
- determining the level of expression of a PDC-specific set of markers in a test cell, and
- comparing the level of expression with a control,
- wherein a level of expression that is approximately identical to the control indicates that the test cell is a dendritic cell.
A method for a modulating plasmacytoid dendritic cell activity comprising
- administering to a plasmacytoid dendritic cell an immunomodulatory agent having a receptor on the surface of the plasinacytoid dendritic cell in an amount effective to modulate plasmacytoid dendritic cell activity, following exposure of the plasmacytoid dendritic cell to an immunostimulatory nucleic acid,
- wherein the receptor on the surface of the plasmacytoid dendritic cell is a PDC-specific marker.
A method of isolating plasmacytoid dendritic cells comprising
- isolating from a bodily sample cells that express at least five PDC-specific markers, and
- removing from the bodily sample cells that express a marker that is not a PDC-specific marker.
A method for identifying a cell as a plasmacytoid dendritic cell comprising
- obtaining a hybridization pattern by hybridizing a nucleic acid sample from a cell to an array of oligonucleotides at known locations on a substrate, and
- comparing the hybridization pattern of the nucleic acid sample to a plasmacytoid expression database,
- wherein the oligonucleotides are complementary to nucleic acid sequences from a plasmacytoid expression database, and
- wherein a hybridization pattern of the nucleic acid sample that is approximately identical to the plasmacytoid expression database indicates that the cell is a plasmacytoid dendritic cell.
A method for identifying a subject responsive to treatment comprising
- determining the level of expression of at least 5 PDC-specific markers in a plasmacytoid dendritic cell population harvested from a subject and exposed to a CpG immunostimulatory nucleic acid, and
- comparing the level of expression of the at least 5 markers in the plasmacytoid dendritic cell population to a control,
- wherein a level of expression that is approximately identical to the control indicates that the subject is responsive to treatment.
A method for evaluating a subject undergoing an immunomodulatory treatment comprising
- determining an in vivo level of expression of a marker in the subject following administration of an immunomodulatory treatment, and
- comparing the in vivo level of expression of the marker with a control,
- wherein an in vivo level of expression of the marker that is approximately identical to the control is indicative of a response to the treatment in vivo.
A method for identifying an agent that modulates plasmacytoid dendritic cell activity comprising
- contacting a plasmacytoid dendritic cell with an agent,
- determining the level of expression of a PDC-specific marker, and
- comparing the level of expression of the PDC-specific marker to a control,
- wherein an agent that induces a level of expression of a PDC-specific marker that is approximately identical or greater than the control is an agent that modulates plasmacytoid dendritic cell activity.
A method of treating a subject to potentiate an immune response induced by administration of an immunostimulatory nucleic acid molecule comprising
- administering to a subject in need thereof an immunostimulatory agent having a receptor on the surface of a plasmacytoid dendritic cell in an amount effective to stimulate a plasmacytoid dendritic cell,
- wherein the agent is a ligand of the receptor or an antibody or antibody fragment specific for the receptor, and
- wherein the receptor on the surface of the plasmacytoid dendritic cell is a PDC-specific marker.
Dependencies are inferred from the claim text.