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US 2003/0148308 A1Published application
Target molecule attachment to surfaces
AssigneeSurModics, Inc.
InventorsRalph A. Chappa, Sheau-Ping Hu, Dale G. Swan, Melvin J. Swanson, Patrick E. Guire
Life of this patent
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Abstract
Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes groups capable of covalently binding to the target molecule. Optionally, the composition can contain photoreactive groups for use in attaching the reagent composition to the surface. The reagent composition can be used to provide activated slides for use in preparing microarrays of nucleic acids.
Claims27
A method for preparing an activated support comprising the steps of:
- a. providing a support;
- and
- b. coupling a reagent composition to the support, wherein the reagent composition comprises a polymeric backbone having more than one thermochemically amine-reactive or sulfhydryl-reactive groups attached thereto, and wherein the reagent composition is configured and arranged to form covalent bonds with functional groups on a target molecule without use of attracting groups to attract the target molecule to the reagent composition.
A method comprising the steps of:
- a. providing a support;
- b. disposing a reagent composition on the support, wherein the reagent composition comprises a polymeric backbone having more than one thermochemically amine-reactive or sulfhydryl-reactive groups attached thereto, and wherein the reagent composition is configured and arranged to form covalent bonds with functional groups on a target molecule without use of attracting groups to attract the target molecule to the reagent composition;
- c. coupling the reagent composition to the support surface to form a reagent composition-coupled support surface;
- and
- d. disposing at least one target molecule on the reagent composition-coupled support surface, wherein the functional groups of the target molecule forms covalent bonds with the reagent composition.
2 more elements
Dependencies are inferred from the claim text.