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US 2003/0148306 A1Published application
Aggrecanase molecules
OwnerMichael J. Agostino, Elizabeth Di Blasio +2inventors
InventorsMichael J. Agostino, Elizabeth Di Blasio, Edward R. LaVallie, Lisa A. Racie
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Abstract
Novel aggrecanase proteins and the nucleotide sequences encoding them as well as processes for producing them are disclosed. Methods for developing inhibitors of the aggrecanase enzymes and antibodies to the enzymes for treatment of conditions characterized by the degradation of aggrecan are also disclosed.
Claims17
An isolated DNA molecule comprising a DNA sequence chosen from:
- a) the sequence of SEQ ID NO. 5 from nucleotide #1-#2270;
- b) the sequence of SEQ ID NO. 7 from nucleotide #1-#2339;
- c) the sequence of SEQ ID NO. 3 from nucleotide #1 to #3899;
- and
- d) the sequence of SEQ ID NO. 9 from nucleotide #1 to #5001;
- e) the sequence of SEQ. ID NO. 11 from nucleotide #1 to #3369;
- and
- f) naturally occurring human allelic sequences and equivalent degenerative codon sequences of (a) through (e).
5 more elements
A purified aggrecanase protein comprising an amino acid sequence chosen from:
- a) the amino acid sequence set forth in SEQ ID NO. 6 from amino acid #1-#756;
- b) the amino acid sequence set forth in SEQ ID NO. 8 from amino acid #1-#779;
- c) the amino acid sequence set forth in SEQ ID NO. 10 from amino acid #1-#1057;
- d) the amino acid sequence set forth in SEQ ID NO. 13 from amino acid #1-#1122;
- and
- e) homologous aggrecanase proteins consisting of addition, substitution, and deletion mutants of the sequences of (a) through (d).
3 more elements
A method for identifying inhibitors of aggrecanase comprising
- a) providing an aggrecanase protein chosen from:
- i) SEQ ID NO. 6 or a fragment thereof;
- ii) SEQ ID NO. 8 or a fragment thereof;
- iii) SEQ. ID NO. 10 or a fragment thereof;
- and
- iv) SEQ. ID NO. 13 or a fragment thereof;
- b) combining the aggrecanase with a potential inhibitor and
- c) evaluating whether the potential inhibitor inhibits aggrecanase activity.
5 more elements
Dependencies are inferred from the claim text.