Method for immobilisation
OwnerZicai Liang, Anil Kumarinventors
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Abstract
A method for immobilisation of nucleic acids onto glass and silicon surfaces is described, wherein said nucleic acids are immobilised onto unmodified glass and other silicon surfaces. A new class of modified nucleic acids, namely silanised nucleic acids, and methods of preparing such modified nucleic acids, as well as a method for producing DNA chips of various density with only end-attachment of DNA applied, are also described.
Claims10
Method of immobilising nucleic acid in solution onto glass and silicon surfaces by applying said solution onto said surfaces, c h a r a c t e r i s e d in that said nucleic acid is silylated by means of a silylating reagent, optionally in the presence of a coupling agent or a bifunctional cross-linking agent, so as to exhibit a reactive silyl group capable of being immobilised onto unmodified glass and other silicon surfaces.
Method of any of the preceding claims, wherein said silylating reagent comprises a reactive group, preferably selected from amino, mercapto, thio, acrylic, cyano, glycidoxy, or any other reactive group capable of reacting with the reactive functionality of the nucleic acid, optionally in the presence of a coupling reagent, or with at least one of the reactive groups of a cross-linking agent.
Method of any of the preceding claims, wherein said coupling agent is selected from the group of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-hydrochloride, 1-cyclohexyl-3-(2-morpholino-ethyl) carbodiimide, dicyclohexylcarbodi-imide, diisopropyl carbodiimide, N-ethyl-3-phenylisoxazolium-3′-sulphonate, N,N′-carbonyldiimidazole.
Method of any of the preceding claims, wherein said bifunctional cross-linking agent is selected from dithiobis(succinimidylpropionate), disuccinimidylsuberate, disuccinimidyl tartrate, bis[2(succinimidooxycarbonyloxy)ethyl]sulphonate, ethyleneglycobis (succinimidylsuccinate), disuccinimidyl glutarate, N,N′-disucciniimidyl carbonate, dimethyladipimidate dihydrochioride, dimethylpimelimidate dihydrochloride, dimethylsuberimidate dihydrochloride, dimethyl-3,3′-dthiobispropionimidate, 1,4di-[3′-(2′-pyridyldithio propionamido]-butane, bismaleimidohexane, 1,5-difluoro-2,4-dinitrobenzene, 1,4-butandiol diglycidyl ether, adipicacid dihydrazide, carbohydrazide, N-succinimidyl-3-(2-pyridyldithio)-propionate, succinimidyl-4-(N-maleimidomethyl)-cyclohexane-1-carboxylate, m-maleimidobenzoyl-N-hydroxysuccinimide ester, N-succinimidyl-(4-iodoacetyl)-aminobenzoate, succinimidyl-4(p-maleimidophenyl)-butyrate, succinimidyl-6-[(iodoacetyl)amino]-hexanoate, succinimidyl-4[((iodoacetyl)-amino)-metyl]-cyclohexane-1-carboxylate, p-nitrophenyliodoacetate, 4-(4-N-maleimido-phenyl)-butyric acid hydrazidehydrochloride, 4-(N-maleimidomethyl)-cyclohexane-1-carboxyl-hydrazidehydrochloride, 3-(2-Pyridyldithio)-propionyl hydrazide.
Method of any of the preceding claims, wherein the solvent is selected from the group of water, DMSO, DMF, and aqueous buffer solutions, or any combination thereof.
The use of the method of any of the preceding claims for the preparation of microarrays.
Dependencies are inferred from the claim text.