Metabolite transporters
AssigneeMonsanto Technology LLC
InventorsHenry E. Valentin, Toni Voelker, Wei Zheng, Thomas J. Savage
Life of this patent
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Abstract
The present invention is in the field of plant genetics and biochemistry. More specifically, the invention relates to genes associated with nucleotide triphosphate transport. The present invention provides and includes nucleic acid molecules, proteins, and antibodies associated with the genes involved in nucleotide triphosphate transport. The present invention also provides methods for utilizing such agents, for example, in gene isolation, gene analysis, and the production of transgenic plants. Moreover, the present invention includes transgenic plants modified to express proteins associated with nucleotide triphosphate transport.
Claims46
A substantially purified nucleic acid molecule that encodes an adenlylate transporter, wherein said nucleic acid molecule comprises SEQ ID NO:
- 1.
A nucleic acid molecule which comprises:
- (A) a promoter region which functions in a plant cell to cause the production of an mRNA molecule;
- (B) an exogenous structural nucleic acid molecule encoding a protein or fragment thereof comprising an amino acid sequence of SEQ ID NO:
- 2;
- and (C) a 3′ non-translated sequence that functions in said plant cell to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA molecule.
A substantially purified protein comprising SEQ ID NO:
- 2.
An antibody capable of specifically binding a substantially purified protein comprising SEQ ID NO:
- 2.
A transformed plant having an exogenous nucleic acid molecule that encodes an adenylate transporter, wherein said nucleic acid molecule comprises an Arabidopsis nucleic acid sequence.
A method of producing a plant having seeds with an increased tocopherol level comprising:
- (A) transforming said plant with a nucleic acid molecule, wherein said nucleic acid molecule comprises SEQ ID NO:
- 1;
- and (B) growing said plant.
A seed derived from a transformed plant having an exogenous nucleic acid molecule comprising a nucleic acid sequence comprising SEQ ID NO:
- 1, wherein said seed has an increased tocopherol level relative to seeds from a plant having a similar genetic background but lacking said exogenous nucleic acid molecule.
Oil derived from a seed of a transformed plant, wherein said transformed plant contains an exogenous nucleic acid molecule comprising a nucleic acid sequence comprising SEQ ID NO:
- 1.
Feedstock comprising a transformed plant or part thereof, wherein said transformed plant has an exogenous nucleic acid molecule that comprises a nucleic acid sequence comprising SEQ ID NO:
- 1.
A meal comprising plant material manufactured from a transformed plant, wherein said transformed plant has an exogenous nucleic acid molecule that comprises a nucleic acid sequence comprising SEQ ID NO:
- 1.
A substantially purified nucleic acid molecule comprising as operably linked components:
- (A) a promoter region which functions in a plant cell to cause the production of an mRNA molecule;
- (B) a nucleic acid sequence that encodes an adenylate transporter or a fragment there of;
- wherein said purified nucleic acid molecule further comprises one or more expression cassettes, each of which expresses a member selected from the group consisting of slr1736, ATPT2, dxs, dxr, GGH, GGPPS, HPPD, MT1, TMT2, GMT, slr 1737, and an antisense construct for homogentisic acid dioxygenase.
A transgenic plant comprising a substantially purified nucleic acid molecule comprising as operably linked components:
- (A) a promoter region which functions in a plant cell to cause the production of an mRNA molecule;
- (B) a nucleic acid sequence that encodes an adenylate transporter or a fragment there of;
- wherein said purified nucleic acid molecule further comprises one or more expression cassettes, each of which expresses a member selected from the group consisting of slr1736, ATPT2, dxs, dxr, GGH, GGPPS, HPPD, MT1, TMT2, GMT, slr 1737, and an antisense construct for homogentisic acid dioxygenase.
Dependencies are inferred from the claim text.