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US 2003/0148287 A1Published application
Libraries and kits for detecting transcription factor activity
OwnerXianqiang Li, Xin Jianginventors
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Abstract
A library of nucleic acid constructs is provided in which each construct comprises a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding the cis element sequence varying within the library of constructs, a promoter sequence 3′ relative to the cis element sequence, a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library and wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs.
Claims80
A library of nucleic acid constructs, each construct comprising:
- a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding, the cis element sequence varying within the library of constructs;
- a promoter sequence 3′ relative to the cis element sequence;
- and
- a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library;
- wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs.
2 more elements
A library of expression vectors comprising:
- a library of constructs, each construct comprising
- a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding, the cis element sequence varying within the library of constructs;
- a promoter sequence 3′ relative to the cis element sequence;
- and
- a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library of constructs;
- wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs.
3 more elements
A library of cells transduced or transfected with a library of constructs, each construct comprising:
- a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding, the cis element sequence varying within the library of constructs;
- a promoter sequence 3′ relative to the cis element sequence;
- and
- a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library;
- wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs.
2 more elements
A kit comprising
- a library of nucleic acid constructs, each construct comprising:
- a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding, the cis element sequence varying within the library of constructs,
- a promoter sequence 3′ relative to the cis element sequence, and
- a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library,
- wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs;
- and
- a library of hybridization probes for detecting by a hybridization assay a plurality of the variable sequences of the reporter sequences comprised in the library of nucleic acid constructs and/or complements of the variable sequences.
4 more elements
A kit comprising:
- a library of expression vectors comprising
- a library of nucleic acid constructs, each construct comprising:
- a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding, the cis element sequence varying within the library of constructs,
- a promoter sequence 3′ relative to the cis element sequence, and
- a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library,
- wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs;
- and
- a library of hybridization probes for detecting by a hybridization assay a plurality of the variable sequences of the reporter sequences comprised in the library of nucleic acid constructs, and/or complements of the variable sequences.
5 more elements
A kit comprising
- a library of cells transduced or transfected with a library of constructs, each construct comprising:
- a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding, the cis element sequence varying within the library of constructs,
- a promoter sequence 3′ relative to the cis element sequence, and
- a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library,
- wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs;
- and
- a library of hybridization probes for detecting by a hybridization assay a plurality of the variable sequences of the reporter sequences comprised in the library of nucleic acid constructs, and/or complements of the variable sequences.
4 more elements
A kit comprising
- a library of nucleic acid constructs, each construct comprising:
- a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding, the cis element sequence varying within the library of constructs,
- a promoter sequence 3′ relative to the cis element sequence, and
- a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library,
- wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs;
- and
- a look-up table, in physical form and/or stored on computer readable media, the look-up table identifying a relationship between the reporter sequences in the library and the cis elements in the library and/or the transcription factors that bind to the cis elements in the library.
4 more elements
A kit comprising:
- a library of expression vectors comprising
- a library of nucleic acid constructs, each construct comprising:
- a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding, the cis element sequence varying within the library of constructs,
- a promoter sequence 3′ relative to the cis element sequence, and
- a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library,
- wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs;
- and
- a look-up table, in physical form and/or stored on computer readable media, the look-up table identifying a relationship between the reporter sequences in the library of constructs and the cis elements in the library of constructs and/or the transcription factors in the library of constructs that bind to the cis elements.
5 more elements
A kit comprising
- a library of cells transduced or transfected with a library of constructs, each construct comprising:
- a cis element sequence comprising one or more copies of a cis element to which a transcription factor is capable of binding, the cis element sequence varying within the library of constructs,
- a promoter sequence 3′ relative to the cis element sequence, and
- a reporter sequence 3′ relative to the promoter sequence that comprises a variable sequence that varies within the library,
- wherein a same cis element sequence is employed with a given reporter sequence within the library of constructs;
- and
- a look-up table, in physical form and/or stored on computer readable media, the look-up table identifying a relationship between the reporter sequences in the library of constructs and the cis elements in the library of constructs and/or the transcription factors in the library of constructs that bind to the cis elements.
4 more elements
Dependencies are inferred from the claim text.