US 2003/0148280 A1Published application

Amplification and detection reagents for HIV-1

AssigneeAbbott Laboratories

InventorsBarbara J. Harris, John R. Hackett JR., Priscilla Swanson, Klara Abravaya, Sushil G. Devare, Jacek J. Gorzowski, Claudia A. Esping, Ning Tang

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Abstract

Oligonucleotide primer sets, probes, and combinations of the primer sets and probes are provided herein. These reagents are useful for amplifying and detecting HIV-1 target sequences in a test sample.

Claims9

Dependent claims

A primer set selected from the group consisting of:

primer set 1 (SEQ. ID. NO. 1 and SEQ. ID. NO. 2);
primer set 2 (SEQ. ID. NO. 3 and SEQ. ID. NO. 2);
primer set 3 (SEQ. ID. NO. 4 and SEQ. ID. NO. 2);
primer set 4 (SEQ. ID. NO. 5 and SEQ. ID. NO. 2);
primer set 5 (SEQ. ID. NO. 6 and SEQ. ID. NO. 2);
primer set 6 (SEQ. ID. NO. 7 and SEQ. ID. NO. 2);
primer set 7 (SEQ. ID. NO. 8 and SEQ. ID. NO. 2);
primer set 8 (SEQ. ID. NO. 9 and SEQ. ID. NO. 10);
primer set 9 (SEQ.ID. NO. 9 and SEQ.ID. NO. 11);
primer set 10 (SEQ. ID. NO. 1 and SEQ. ID. NO. 12);
primer set 11 (SEQ. ID. NO. 13 and SEQ. ID. NO. 14);
primer set 12 (SEQ. ID. NO. 13 and SEQ. ID. NO. 15);
primer set 13 (SEQ. ID. NO. 13 and SEQ. ID. NO. 2);
primer set 14 (SEQ. ID. NO. 9 and SEQ. ID. NO. 12);
primer set 15 (SEQ. ID. NO. 1 and SEQ. ID. NO. 11);
primer set 16 (SEQ. ID. NO. 16 and SEQ. ID. NO. 12);
primer set 17 (SEQ. ID. NO. 16 and SEQ. ID. NO. 17);
primer set 18 (SEQ. ID. NO. 3 and SEQ. ID. NO. 12);
primer set 19 (SEQ. ID. NO. 3 and SEQ. ID. NO. 18);
primer set 20 (SEQ. ID. NO. 19 and SEQ. ID. NO. 18);
primer set 21 (SEQ. ID. NO. 13 and SEQ. ID. NO. 17);
primer set 22 (SEQ. ID. NO. 13 and SEQ. ID. NO. 20);
primer set 23 (SEQ. ID. NO. 21 and SEQ. ID. NO. 18);
primer set 24 (SEQ. ID. NO. 21 and SEQ. ID. NO. 14);
primer set 25 (SEQ. ID. NO. 21 and SEQ. ID. NO. 20);
primer set 26 (SEQ. ID. NO. 4 and SEQ. ID. NO. 20);
primer set 27 (SEQ. ID. NO. 5 and SEQ. ID. NO. 15);
primer set 28 (SEQ. ID. NO. 21 and SEQ. ID. NO. 22);
primer set 29 (SEQ. ID. NO. 21 and SEQ. ID. NO. 23);
primer set 30 (SEQ. ID. NO. 5 and SEQ. ID. NO. 23);
primer set 31 (SEQ. ID. NO. 28 and SEQ. ID. NO. 29);
primer set 32 (SEQ. ID. NO. 28 and SEQ. ID. NO. 30);
primer set 33 (SEQ. ID. NO. 28 and SEQ. ID. NO. 31);
primer set 38 (SEQ. ID. NO. 37 and SEQ. ID. NO. 32);
primer set 39 (SEQ. ID. NO. 37 and SEQ. ID. NO. 33);
primer set 40 (SEQ. ID. NO. 38 and SEQ. ID. NO. 29);
primer set 41 (SEQ. ID. NO. 38 and SEQ. ID. NO. 30);
primer set 42 (SEQ. ID. NO. 4 and SEQ. ID. NO. 22);
primer set 43 (SEQ. ID. NO. 4 and SEQ. ID. NO. 40);
primer set 44 (SEQ. ID. NO. 34 and SEQ. ID. NO. 22);
primer set 45 (SEQ. ID. NO. 34 and SEQ. ID. NO. 40);
primer set 46 (SEQ. ID. NO. 24 and SEQ. ID. NO. 25);
and primer set 47 (SEQ. ID. NO. 26 and SEQ. ID. NO. 27).

40 more elements

An oligonucleotide set for comprising a primer set and detection probe, wherein the oligonucleotide set is selected from the group consisting of:

oligo set 1 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 41);
oligo set 2 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 42);
oligo set 3 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 43);
oligo set 4 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 44);
oligo set 5 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 45);
oligo set 7 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 47);
oligo set 8 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 48);
oligo set 9 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 49);
oligo set 10 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 50);
oligo set 11 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 51);
oligo set 12 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 52);
oligo set 13 (SEQ. ID. NO. 28, SEQ. ID. NO. 29, and SEQ. ID. NO. 53);
oligo set 14 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 41);
oligo set 15 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 42);
oligo set 16 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 43);
oligo set 17 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 44);
oligo set 18 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 45);
oligo set 20 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 47);
oligo set 21 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 48);
oligo set 22 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 49);
oligo set 23 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 50);
oligo set 24 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 51);
oligo set 25 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 52);
oligo set 26 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 53);
oligo set 27 (SEQ. ID. NO. 28, SEQ. ID. NO. 31, and SEQ. ID. NO. 41);
oligo set 28 (SEQ. ID. NO. 28, SEQ. ID. NO. 31, and SEQ. ID. NO. 42);
oligo set 29 (SEQ. ID. NO. 28, SEQ. ID. NO. 31, and SEQ. ID. NO. 43);
oligo set 30 (SEQ. ID. NO. 28, SEQ. ID. NO. 31, and SEQ. ID. NO. 44);
oligo set 31 (SEQ. ID. NO. 28, SEQ. ID. NO. 31, and SEQ. ID. NO. 45);
oligo set 33 (SEQ. ID. NO. 28, SEQ. ID. NO. 31, and SEQ. ID. NO. 47);
oligo set 34 (SEQ. ID. NO. 28, SEQ. ID. NO. 31, and SEQ. ID. NO. 48);
oligo set 35 (SEQ. ID. NO. 37, SEQ. ID. NO. 32, and SEQ. ID. NO. 55);
oligo set 36 (SEQ. ID. NO. 37, SEQ. ID. NO. 33, and SEQ. ID. NO. 55);
oligo set 38 (SEQ. ID. NO. 37, SEQ. ID. NO. 33, and SEQ. ID. NO. 57);
oligo set 39 (SEQ. ID. NO. 38, SEQ. ID. NO. 29, and SEQ. ID. NO. 50);
oligo set 40 (SEQ. ID. NO. 38 , SEQ. ID. NO. 29, and SEQ. ID. NO. 51);
oligo set 41 (SEQ. ID. NO. 38, SEQ. ID. NO. 29, and SEQ. ID. NO. 52);
oligo set 42 (SEQ. ID. NO. 38, SEQ. ID. NO. 29, and SEQ. ID. NO. 53);
oligo set 43 (SEQ. ID. NO. 38, SEQ. ID. NO. 30, and SEQ. ID. NO. 50);
oligo set 44 (SEQ. ID. NO. 38, SEQ. ID. NO. 30, and SEQ. ID. NO. 51);
oligo set 45 (SEQ. ID. NO. 38, SEQ. ID. NO. 30, and SEQ. ID. NO. 52);
oligo set 46 (SEQ. ID. NO. 38, SEQ. ID. NO. 30, and SEQ. ID. NO. 53);
oligo set 47 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 58);
oligo set 48 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 59);
oligo set 49 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 60);
oligo set 50 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 61);
oligo set 51 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 62);
oligo set 52 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 63);
oligo set 53 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 64);
and oligo set 54 (SEQ. ID. NO. 28, SEQ. ID. NO. 30, and SEQ. ID. NO. 65).

47 more elements

Dependencies are inferred from the claim text.

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