Sandwich assays for collagen type i fragments
AssigneeOsteometer Biotech A/S
InventorsCHRISTIAN ROSENQUIST, PER QVIST
Life of this patent
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Abstract
Sandwich assays for collagen degradation products are conducted using an antibody of the same specificity on both sides of the sandwich or using a first antibody reactive with an epitope contained in the sequence EKAHDGGR and a second antibody which may be the same or different.
Claims13
A method of measurement of the rate of type 1 collagen resorption comprising measuring in a sample the amount of a population of collagen fragments by a sandwich assay using a first antibody reactive with a first epitope located in the collagen amino acid sequence EKAHDGGR or in isomerised and/or racemised variants thereof and a second antibody reactive with a second collagen epitope located in said fragments.
A method as claimed in any preceding claim, wherein the assay is a sandwich radio-immune assay or a sandwich ELISA assay.
A method as claimed in any preceding claim, wherein the molecular weight of fragments detected in the assay exceeds 1500 Da.
A method as claimed in any preceding claim, wherein the molecular weight of fragments detected in the assay exceeds 25000 Da.
A method of conducting a sandwich assay comprising:
- mixing a target antigen containing at least two antigenically similar epitopes with a first antibody reactive with both said epitopes, which first antibody is coupled to a capture moiety and with a second antibody reactive with both said epitopes, which second antibody is coupled to a label, so as to form a first antibody—target antigen—second antibody sandwich,
- capturing said sandwich to a capture substrate having an affinity for said capture moiety of said first antigen,
- and detecting the capture of said sandwich by detection of the label of the second antibody.
A sandwich assay for collagen degradation products in which antibodies of essentially identical specificity are used on both sides of the sandwich.
A method of measurement of the concentration of collagen degradation products in a sample comprising conducting a sandwich assay using first and second immunological binding partners (which may be the same or different) each immunologically reactive an epitope in an N-terminal telopeptide fragment produced upon collagen degradation in vivo.
Dependencies are inferred from the claim text.