Genes from the 20q13 amplicon and their uses
AssigneeThe Regents of the University of California
InventorsJOE W. GRAY, COLIN COLLINS, SOO-IN HWANG, TONY GODFREY, DAVID KOWBEL, JOHANNA ROMMENS
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Abstract
The present invention relates to cDNA sequences from a region of amplification on chromosome 20 associated with disease. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases. The sequences can also be used for treatment of diseases.
Claims44
An isolated nucleic acid molecule comprising a polynucleotide sequence having a subsequence which specifically hybridizes under stringent conditions to a sequence selected from the group consisting of SEQ. ID. No. 2, SEQ. ID. No. 3, SEQ. ID. No. 4, SEQ. ID. No. 5, SEQ. ID. No. 6, SEQ. ID. No. 7, SEQ. ID. No. 8, SEQ. ID. No. 9, SEQ. ID. No. 10, and SEQ. ID. No. 12.
The isolated nucleic acid of claim 14, 16, 18, 20, wherein the subsequence is SEQ. ID. No. 8.
A method of screening for neoplastic cells in a sample, the method comprising:
- contacting a nucleic acid sample from a human patient with a probe which hybridizes selectively to a target polynucleotide sequence comprising a sequence selected from the group consisting of SEQ. ID. No. 1, SEQ. ID. No. 2, SEQ. ID. No. 3, SEQ. ID. No. 4, SEQ. ID. No. 5, SEQ. ID. No. 6, SEQ. ID. No. 7, and SEQ. ID. No. 8, wherein the probe is contacted with the sample under conditions in which the probe hybridizes selectively with the target polynucleotide sequence to form a stable hybridization complex;
- and
- detecting the formation of a hybridization complex.
A method for detecting a neoplastic cell in a biological sample, the method comprising:
- contacting the sample with an antibody that specifically binds a polypeptide antigen encoded by a polynucleotide sequence comprising a sequence selected from the group consisting of SEQ. ID. No. 1, SEQ. ID. No. 2, SEQ. ID. No. 3, SEQ. ID. No.4, SEQ. ID. No. 5, SEQ. ID. No.6, SEQ. ID. No. 7, SEQ. ID. No. 8, SEQ. ID. No. 9, SEQ. ID. No. 10, and SEQ. ID. No. 12;
- and
- detecting the formation of an antigen-antibody complex.
A method of inhibiting the pathological proliferation of cancer cells, the method comprising inhibiting the activity of a gene product of an endogenous gene having a subsequence which hybridizes under stringent conditions to a sequence selected from the group consisting of SEQ. ID. 1, SEQ. ID. No. 2, SEQ. ID. No. 3, SEQ. ID. No. 4, SEQ. ID. No. 5, SEQ. ID. No. 6, SEQ. ID. No. 7, SEQ. ID. No. 8, SEQ. ID. NO. 9, SEQ. ID. NO. 10, and SEQ. ID. No. 12.
A method of detecting a cancer, said method comprising detecting the overexpression of a protein encoded in a 20q13 amplicon.
Dependencies are inferred from the claim text.