In vivo monitoring method of transgenic plants and system using the same
AssigneeDongbu Hannong Chemical Co., Ltd.
InventorsJoo-Kon Kim, Byoung-Chull Chung, Yong-Joo Park
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Abstract
The present invention relates to a method for visualizing GFP expression in callus, various tissue and organ of the transgenic plants as image and system using the same. The said method needs no other additional genetic product, substrate or cofactor and can detect very simply and quickly GFP expression by using the said system of the present invention consisting of a CCD camera, a light source, band-pass filter and data processing computer, so it provides many advantages for selection of transgenic seeds, for studying of gene expression in the tissue or organ of plants, or for studying of specificity of each development step.
Claims2
A fluorometry method for monitoring the transformation of plants based on the in vivo expression of a heterologous green fluorescent protein, comprising the steps:
- projecting excitation light from a light source through a blue bandpass filter onto a plant sample at an angle of 45°, said blue bandpass filter passing light ranging, in wavelength, from 470 to 490, said excitation light having a wavelength of around 488 nm with a peak at 480 nm;
- detecting light generated from the plant sample by use of a charge coupled device color video camera equipped with a zoom lens, which is positioned on the axis vertical to the plane of the sample, said light passing through a green bandpass filter which passes light ranging, in wavelength, from 500 to 550 nm before arriving at said zoom lens, so as to have a wavelength of around 509 nm, and photographing the image of the plant sample on the basis of the light generated from the plant sample;
- and
- processing the image in a computer to determine whether the plant sample is transgenic or not.
Dependencies are inferred from the claim text.