Nanoparticle delivery vehicle

A nanoparticle delivery vehicle comprising:

• (a) a plurality of different targeting agents;
• (b) a nanoparticle scaffold;
• and
• (c) an active agent.

A method of modulating the expression of a target nucleic acid sequence, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a nuclear localization signal and an active agent capable of interacting with a target nucleic acid sequence whose expression is to be modulated;
• (b) contacting a target cell comprising a target nucleic acid sequence with the nanoparticle delivery vehicle;
• and
• (c) modulating the expression of the target nucleic acid sequence through the contacting of step (b), whereby the expression of a target nucleic acid sequence is modulated.

A method of modulating the expression of a target nucleic acid sequence, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a plurality of targeting agents and an active agent capable of interacting with a target nucleic acid sequence whose expression is to be modulated;
• (b) contacting a target cell comprising a target nucleic acid sequence with the nanoparticle delivery vehicle;
• and
• (c) modulating the expression of the target nucleic acid sequence through the contacting of step (b), whereby the expression of a target nucleic acid sequence is modulated.

A method of modulating the expression of a target protein, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a nuclear localization signal and a single stranded antisense nucleic acid sequence complementary to a nucleic acid sequence encoding a target protein;
• (b) contacting a target cell comprising a nucleic acid sequence encoding a target protein with the nanoparticle delivery vehicle;
• and
• (c) modulating the expression of the target protein through the contacting of step (b), whereby the expression of a target protein is modulated.

A method of modulating the expression of a target protein, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a plurality of targeting agents and a single stranded antisense nucleic acid sequence complementary to a nucleic acid sequence encoding a target protein;
• (b) contacting a target cell comprising a nucleic acid sequence encoding a target protein with the nanoparticle delivery vehicle;
• and
• (c) modulating the expression of the target protein through the contacting of step (b), whereby the expression of a target protein is modulated.

A method of modulating transcription in a sample, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a nuclear localization signal and an active agent comprising a ligand for which a wild-type transcription component has greater affinity than a natural ligand of the wild-type transcription component;
• (b) contacting a sample comprising the wild-type transcription component with the nanoparticle delivery vehicle;
• and
• (c) modulating transcription in the sample through the contacting of step (b), wherein transcription in a sample is modulated.

A method of modulating transcription in a sample, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a plurality of targeting agents and an active agent comprising a ligand for which a wild-type transcription component has greater affinity than a natural ligand of the wild-type transcription component;
• (b) contacting a sample comprising the wild-type transcription component with the nanoparticle delivery vehicle;
• and
• (c) modulating transcription in the sample through the contacting of step (b), wherein transcription in a sample is modulated.

A method of modulating RNA splicing in a sample, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a nuclear localization signal and a nucleic acid sequence known or suspected to alter the splicing pattern for a target gene;
• and
• (b) contacting a sample comprising the target gene with the nanoparticle delivery vehicle;
• and
• (c) modulating RNA splicing in a sample through the contacting of step (b), whereby RNA splicing in a sample is modulated.

A method of modulating RNA splicing in a sample, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a plurality of targeting agents and a nucleic acid sequence known or suspected to alter the splicing pattern for a target gene;
• and
• (b) contacting a sample comprising the target gene with the nanoparticle delivery vehicle;
• and
• (c) modulating RNA splicing in a sample through the contacting of step (b), whereby RNA splicing in a sample is modulated.

A method of modulating the translation of an mRNA sequence encoding a protein of interest, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a nuclear localization signal and a single stranded nucleic acid sequence complementary to a nucleic acid sequence of an mRNA sequence encoding a protein of interest;
• (b) contacting a sample comprising the mRNA sequence encoding a protein of interest with the nanoparticle delivery vehicle;
• and
• (c) modulating the translation of an mRNA sequence encoding a protein of interest through the contacting of step (b), whereby the translation of an mRNA sequence encoding a protein of interest is modulated.

A method of modulating the translation of an mRNA sequence encoding a protein of interest, the method comprising:

• (a) providing a nanoparticle delivery vehicle comprising a nanoparticle, a plurality of targeting agents and a single stranded nucleic acid sequence complementary to a nucleic acid sequence of an mRNA sequence encoding a protein of interest;
• (b) contacting a sample comprising the mRNA sequence encoding a protein of interest with the nanoparticle delivery vehicle;
• and
• (c) modulating the translation of an mRNA sequence encoding a protein of interest through the contacting of step (b), whereby the translation of an mRNA sequence encoding a protein of interest is modulated.