Chlamydia antigens and corresponding dna fragments and uses thereof
AssigneeAventis Pasteur Limited/Aventis Pasteur Limitee
InventorsANDREW D. MURDIN, RAYMOND P. OOMEN
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Abstract
In summary of this disclosure, the present invention provides a method of nucleic acid, including DNA, immunization of a host, including humans, against disease caused by infection by a strain of Chlamydia, specifically C. pneumoniae, employing a vector, containing a nucleotide sequence encoding a CPN100149 polypeptide of a strain of Chlamydia pneumoniae and a promoter to effect expression of the CPN100149 polypeptide in the host. Modifications are possible within the scope of this invention.
Claims37
An isolated polynucleotide selected from the group consisting of:
- (a) a polynucleotide having a sequence comprising the nucleotide sequence SEQ ID NO:1, and functional fragments thereof;
- (c) a polynucleotide encoding a polypeptide having a sequence that is at least 75% homologous to SEQ ID NO:2, and functional fragments thereof;
- and
- (d) a polynucleotide capable of hybridizing under stringent conditions to a polynucleotide having a sequence comprising the nucleotide sequence SEQ ID NO:1, and functional fragments thereof.
An isolated polypeptide having a sequence that is at least 75% homologous to SEQ ID NO:4, and functional fragments thereof
A method for detecting the presence of Chlamydia in a sample comprising the steps of:
- (a) contacting said sample with a detecting reagent that binds to CPN100149 polypeptide to form a complex;
- and
- (b) detecting said formed complex.
An affinity chromatography method for substantially purifying a CPN100149 polypeptide, comprising the steps of:
- (a) contacting a sample containing a CPN100149 polypeptide with a detecting reagent that binds to CPN100149 polypeptide to form a complex;
- (c) isolating said formed complex;
- (c) dissociating said formed complex;
- and
- (d) isolating the dissociated CPN100149 polypeptide.
Dependencies are inferred from the claim text.